Q1RGU4 (ALR_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	RBE_1339

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Alanine racemase HAMAP-Rule MF_01201		PRO_0000272404
Regions
	Domain	28 – 76	49	RPE1 insert
Sites
	Active site	83	1	Proton acceptor; specific for D-alanine By similarity
	Active site	305	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	353	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	83	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RGU4 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 91E898D4A3B5E1B6	FASTA	410	45,476
	10 20 30 40 50 60 MSLCTLEINL SAIKANYRLL KNICENSVDF LHNVANKEEF AGNTSPRTAA YTLVREDASL 70 80 90 100 110 120 GSTPKLPLGA SYAKNLLVGA AVKANSYGLG AIEISKTLLE ENCRYFFVAS SNEGISLRKA 130 140 150 160 170 180 IGDEVNILIL NGVFEHDALE LIEYNLIPVL NNLNQIKIWQ KFSNLKNQLL PCYLHFNTGI 190 200 210 220 230 240 NRLGLNHNEI EQLINNRDLL KGLNVEYIIS HLSASEDSDN PYNLEQLNKF KAYLEYFPNA 250 260 270 280 290 300 KASLANSGGI FLGKDYHFNL VRPGAALYGL NPLGSNKPNP MQNPVTLKAP IIHLQNLTYG 310 320 330 340 350 360 SRIGYNMTFT TKRDSLIATL PFGYADGFSR NFSNQGTVFI NSRNVPIIGR ISMDLVNIDV 370 380 390 400 410 TDLPPSDIFL GQEVEIIGNN CTPDKIADII GTIGYEILTS LGNRYKRIYT

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05420.1.
RefSeq	YP_538509.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RGU4.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_1339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE05420; ABE05420; RBE_1339.
GeneID	3995819.
KEGG	rbe:RBE_1339.
PATRIC	17884328. VBIRicBel102610_1512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031445.
KO	K01775.
OMA	YCTPDKI.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-1377-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase. Divergent sequence.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR005728. Rickett_RPE. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit. TIGR01045. RPE1. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_RICBR

Accession

Primary (citable) accession number: Q1RGU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program