Dihydrofolate reductase - Escherichia coli (strain UTI89 / UPEC)

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Q1RGF0 (Q1RGF0_ECOUT) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 40. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Submitted name:
Dihydrofolate reductase EMBL ABE05564.1
EC=1.5.1.3 EMBL ABE05564.1

Gene names

Name:	folA EMBL ABE05564.1
Ordered Locus Names:	UTI89_C0054

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP] EMBL ABE05564.1

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	NADP PDB 3QL0 Nucleotide-binding PDB 3QL0
Molecular function	Oxidoreductase EMBL ABE05564.1
Technical term	3D-structure PDB 3QL0 Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

3

Nucleotide binding

3

Nucleotide binding

3

Sites

Binding site

1

NADP; via amide nitrogen and carbonyl oxygen PDB 3QL0

Binding site

1

NADP; via carbonyl oxygen PDB 3QL0

Binding site

1

NADP; via amide nitrogen and carbonyl oxygen PDB 3QL0

Binding site

1

Sequences

Sequence

Length

Mass (Da)

Tools

Q1RGF0 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 6385987DDF0954D4
Show »

204

23,212

        10         20         30         40         50         60 
MRRVQGESVD SPAEYKIFLN IILAPVDDGL RFTYSGDNFF YREISMISLI AALAVDRVIG 

        70         80         90        100        110        120 
MENAMPWNLP ADLAWFKRNT LNKPVIMGRH TWESIGRPLP GRKNIILSSQ PGTDDRVTWV 

       130        140        150        160        170        180 
KSVDEAIAAC GDVPEIMVIG GGRVYEQFLP KAQKLYLTHI DAEVEGDTHF PDYEPDDWES 

       190        200 
VFSEFHDADA QNSHSYCFEI LERR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis." Bhabha G., Lee J., Ekiert D.C., Gam J., Wilson I.A., Dyson H.J., Benkovic S.J., Wright P.E. Science 332:234-238(2011) [PubMed: 21474759] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 46-204 IN COMPLEX WITH NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000243 Genomic DNA. Translation: ABE05564.1.

RefSeq

YP_539095.1. NC_007946.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3QL0	X-ray	1.60	A	46-204	[»]

ProteinModelPortal

SMR

Q1RGF0. Positions 46-204.

ModBase

Protein-protein interaction databases

STRING

Protocols and materials databases

StructuralBiologyKnowledgebase

Genome annotation databases

EnsemblBacteria

EBESCT00000069726; EBESCP00000067204; EBESCG00000068773.

GeneID

GenomeReviews

Gene locus UTI89_C0054 in contig CP000243_GR.

KEGG

eci:UTI89_C0054.

PATRIC

18449805. VBIEscCol42261_0184.

Organism-specific databases

CMR

Phylogenomic databases

eggNOG

GeneTree

EBGT00050000010807.

HOGENOM

OMA

ProtClustDB

Family and domain databases

InterPro

IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]

Gene3D

G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit.

KO

Pfam

PF00186. DHFR_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000194. DHFR. 1 hit.

PRINTS

PR00070. DHFR.

SUPFAM

SSF53597. SSF53597. 1 hit.

PROSITE

PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

Q1RGF0_ECOUT

Accession

Primary (citable) accession number: Q1RGF0

Entry history

Integrated into UniProtKB/TrEMBL:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info