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Protein

Uridylate kinase

Gene

pyrH

Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57UMP; via amide nitrogenUniRule annotation1
Binding sitei58ATP; via amide nitrogenUniRule annotation1
Binding sitei62ATPUniRule annotation1
Binding sitei77UMPUniRule annotation1
Binding sitei165ATPUniRule annotation1
Binding sitei171ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei174ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 18ATPUniRule annotation4
Nucleotide bindingi138 – 145UMPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Ordered Locus Names:UTI89_C0186
OrganismiEscherichia coli (strain UTI89 / UPEC)
Taxonomic identifieri364106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001952 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000539231 – 241Uridylate kinaseAdd BLAST241

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1RG18.
SMRiQ1RG18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 28Involved in allosteric activation by GTPUniRule annotation6

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000047187.
KOiK09903.
OMAiPIIVFDM.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1RG18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNAKPVYK RILLKLSGEA LQGTEGFGID ASILDRMAQE IKELVELGIQ
60 70 80 90 100
VGVVIGGGNL FRGAGLAKAG MNRVVGDHMG MLATVMNGLA MRDALHRAYV
110 120 130 140 150
NARLMSAIPL NGVCDSYSWA EAISLLRNNR VVILSAGTGN PFFTTDSAAC
160 170 180 190 200
LRGIEIEADV VLKATKVDGV FTADPAKDPT ATMYEQLTYS EVLEKELKVM
210 220 230 240
DLAAFTLARD HKLPIRVFNM NKPGALRRVV MGEKEGTLIT E
Length:241
Mass (Da):25,970
Last modified:May 16, 2006 - v1
Checksum:i82EFA75F7226E201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000243 Genomic DNA. Translation: ABE05696.1.
RefSeqiWP_000224573.1. NC_007946.1.

Genome annotation databases

EnsemblBacteriaiABE05696; ABE05696; UTI89_C0186.
KEGGieci:UTI89_C0186.

Similar proteinsi

Entry informationi

Entry nameiPYRH_ECOUT
AccessioniPrimary (citable) accession number: Q1RG18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: October 25, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families