Q1RF60 (HEMH_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	UTI89_C0503

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subunit structure	Monomer By similarity. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ferrochelatase HAMAP MF_00323		PRO_1000019296
Sites
	Metal binding	194	1	Iron By similarity
	Metal binding	275	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RF60 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 79E6F8BF465B1E7E	FASTA	320	35,942
	10 20 30 40 50 60 MRQTKTGILL ANLGTPDAPT PEAVKRYLKQ FLSDRRVVDT SRLLWWPLLR GVILPLRSPR 70 80 90 100 110 120 VAKLYASVWM EGGSPLMVYS RQQQQALAQR LPETPVALGM SYGSPSLESA VDELLAEHVD 130 140 150 160 170 180 HIVVLPLYPQ YSCSTVGAVW DELARILARK RSIPGISFIR DYADNHDYIN ALANSVRASF 190 200 210 220 230 240 AKHGEPDLLL LSYHGIPQRY ADEGDDYPQR CRTTTRELAS ALEMAPEKVM MTFQSRFGRE 250 260 270 280 290 300 PWLMPYTDET LKMLGEKGVG HIQVMCPGFA ADCLETLEEI AEQNREVFLG AGGKKYEYIP 310 320 ALNATPEHIE MMANLVAAYR

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE06004.1.
RefSeq	YP_539535.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1RF60.
SMR	Q1RF60. Positions 3-319.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RF60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000067030; EBESCP00000064508; EBESCG00000066077.
GeneID	3991671.
GenomeReviews	Gene locus UTI89_C0503 in contig CP000243_GR.
KEGG	eci:UTI89_C0503.
PATRIC	18450675. VBIEscCol42261_0609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
GeneTree	EBGT00050000011828.
HOGENOM	HBG697135.
OMA	TIEEIGM.
ProtClustDB	PRK00035.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C0503-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_ECOUT

Accession

Primary (citable) accession number: Q1RF60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program