Reviewed, UniProtKB/Swiss-Prot Q1REH4 (GAL1_ECOUT)

Names and origin

Protein names

Recommended name:
    Galactokinase
    EC=2.7.1.6
Alternative name(s):
    Galactose kinase

Gene names

Name:	galK
Ordered Locus Names:	UTI89_C0754

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP MF_00246
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate phosphorylation Inferred from electronic annotation. Source: InterPro galactose metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP galactokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Galactokinase HAMAP MF_00246		PRO_1000005750
Regions
	Nucleotide binding	122 – 132	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1REH4-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: BE4B6CE6FC48F9A8	FASTA	382	41,476
	10 20 30 40 50 60 MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD 70 80 90 100 110 120 DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN 130 140 150 160 170 180 VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL 190 200 210 220 230 240 GKKDHALLID CRSLGTKAVS MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP 250 260 270 280 290 300 ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS 310 320 330 340 350 360 MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALFPEELVP AVQQAVAEQY 370 380 EAKTGIKETF YVCKPSQGAG QC

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000243 Genomic DNA. Translation: ABE06240.1.
RefSeq	YP_539771.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3991531.
GenomeReviews	Gene locus UTI89_C0754 in contig CP000243_GR.
KEGG	eci:UTI89_C0754.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1REH4.
OMA	Q1REH4. YNDGFVM.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C0754-MON.
Family and domain databases
HAMAP	MF_00246. [Tree]
InterPro	IPR000705. Galactokinase. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal_bd. IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR006206. Mev_galkinase. IPR014721. Ribosomal_S5_D2-type_fold. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000530. Galactokinase. 1 hit.
PRINTS	PR00473. GALCTOKINASE. PR00959. MEVGALKINASE.
TIGRFAMs	TIGR00131. gal_kin. 1 hit.
PROSITE	PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL1_ECOUT

Accession

Primary (citable) accession number: Q1REH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)