Reviewed, UniProtKB/Swiss-Prot Q1REB8 (RLMF_ECOUT)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase F
    EC=2.1.1.48
Alternative name(s):
    23S rRNA mA1618 methyltransferase
    rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	UTI89_C0810

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine. HAMAP MF_01848
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349909

Sequences

	Sequence		Length	Mass (Da)
	Q1REB8-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 10D0EA4C2F3E23D3	FASTA	308	34,180
	10 20 30 40 50 60 MSAQKPGLHP RNRHHSRYDL ATLCQVNPEL RQFLTLTPAG EQSVDFANPL AVKALNKALL 70 80 90 100 110 120 AHFYAVANWD IPDGFLCPPV PGRADYIHHL ADLLAEASGT IPANASILDI GVGANCIYPL 130 140 150 160 170 180 IGVHEYGWRF TGSETSSQAL SSAQAIISAN PGLNRAIRLR RQKESGAIFN GIIHKNEQYD 190 200 210 220 230 240 ATLCNPPFHD SAAAARAGSE RKRRNLGLNK DDALNFGGQQ QELWCEGGEV AFIKKMIEES 250 260 270 280 290 300 KGFAKQVMWF TSLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMNDEQR RRFVNRQR

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000243 Genomic DNA. Translation: ABE06296.1. Different initiation.
RefSeq	YP_539827.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3994497.
GenomeReviews	Gene locus UTI89_C0810 in contig CP000243_GR.
KEGG	eci:UTI89_C0810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1REB8.
Family and domain databases
HAMAP	MF_01848. [Tree]
InterPro	IPR010286. SAM-Mtase_prd. [Graphical view]
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMF_ECOUT

Accession

Primary (citable) accession number: Q1REB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 17 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)