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Q1RCY4

- RUSA_ECOUT

UniProt

Q1RCY4 - RUSA_ECOUT

Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumBy similarity
    Metal bindingi72 – 721MagnesiumBy similarity
    Metal bindingi91 – 911MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL364106:GHPQ-1287-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Ordered Locus Names:UTI89_C1298
    OrganismiEscherichia coli (strain UTI89 / UPEC)
    Taxonomic identifieri364106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001952: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000324833Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi364106.UTI89_C1298.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1RCY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 164DNA bindingBy similarity
    Regioni66 – 738DNA bindingBy similarity

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Phylogenomic databases

    eggNOGiCOG4570.
    HOGENOMiHOG000119986.
    KOiK01160.
    OMAiRGIHYIS.
    OrthoDBiEOG66HVH9.

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001007. RusA. 1 hit.
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1RCY4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG    50
    LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV 100
    KMPVTKGGRL ELTITEMGNE 120
    Length:120
    Mass (Da):13,846
    Last modified:May 16, 2006 - v1
    Checksum:i38401669E8819EA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000243 Genomic DNA. Translation: ABE06780.1.
    RefSeqiYP_540311.1. NC_007946.1.

    Genome annotation databases

    EnsemblBacteriaiABE06780; ABE06780; UTI89_C1298.
    GeneIDi3989960.
    KEGGieci:UTI89_C1298.
    PATRICi18452213. VBIEscCol42261_1359.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000243 Genomic DNA. Translation: ABE06780.1 .
    RefSeqi YP_540311.1. NC_007946.1.

    3D structure databases

    ProteinModelPortali Q1RCY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 364106.UTI89_C1298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE06780 ; ABE06780 ; UTI89_C1298 .
    GeneIDi 3989960.
    KEGGi eci:UTI89_C1298.
    PATRICi 18452213. VBIEscCol42261_1359.

    Phylogenomic databases

    eggNOGi COG4570.
    HOGENOMi HOG000119986.
    KOi K01160.
    OMAi RGIHYIS.
    OrthoDBi EOG66HVH9.

    Enzyme and pathway databases

    BioCyci ECOL364106:GHPQ-1287-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001007. RusA. 1 hit.
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
      Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
      Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: UTI89 / UPEC.

    Entry informationi

    Entry nameiRUSA_ECOUT
    AccessioniPrimary (citable) accession number: Q1RCY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3