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Q1RAR4

- CMOA_ECOUT

UniProt

Q1RAR4 - CMOA_ECOUT

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Protein

tRNA (cmo5U34)-methyltransferase

Gene
cmoA, UTI89_C2074
Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391S-adenosyl-L-methionine By similarity
Binding sitei89 – 891S-adenosyl-L-methionine By similarity
Binding sitei132 – 1321S-adenosyl-L-methionine By similarity
Binding sitei199 – 1991Substrate Reviewed prediction

GO - Molecular functioni

  1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA (uracil) methyltransferase activity Source: InterPro

GO - Biological processi

  1. tRNA wobble uridine modification Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL364106:GHPQ-2057-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cmo5U34)-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:cmoA
Ordered Locus Names:UTI89_C2074
OrganismiEscherichia coli (strain UTI89 / UPEC)
Taxonomic identifieri364106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001952: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247tRNA (cmo5U34)-methyltransferaseUniRule annotationPRO_0000314326Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi364106.UTI89_C2074.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi22 – 3514
Helixi39 – 5315
Beta strandi59 – 635
Turni66 – 683
Helixi69 – 768
Beta strandi80 – 823
Beta strandi84 – 907
Helixi92 – 10312
Beta strandi111 – 1166
Turni118 – 1203
Beta strandi125 – 1339
Helixi135 – 1373
Helixi140 – 1423
Helixi143 – 15311
Beta strandi154 – 16613
Helixi171 – 18717
Helixi190 – 1923
Helixi196 – 20510
Helixi211 – 22111
Beta strandi224 – 2329
Beta strandi235 – 2417

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GEKX-ray1.50A/G1-247[»]
ProteinModelPortaliQ1RAR4.
SMRiQ1RAR4. Positions 20-242.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 663S-adenosyl-L-methionine binding By similarity
Regioni117 – 1182S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000218457.
KOiK15256.
OMAiYDLGCSR.
OrthoDBiEOG628F44.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. tRNA_methyltr_CmoA.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1RAR4-1 [UniParc]FASTAAdd to Basket

« Hide

MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA    50
ERFVQPGTQV YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR 100
HIDAYKAPTP VDVIEGDIRD IAIENASMVV LNFTLQFLEP SERQALLDKI 150
YQGLNPGGAL VLSEKFSFED AKVGELLFNM HHDFKRANGY SELEISQKRS 200
MLENVMLTDS VETHKARLHK AGFEHSELWF QCFNFGSLVA LKAEDAA 247
Length:247
Mass (Da):27,791
Last modified:May 16, 2006 - v1
Checksum:i2930565165A0A7F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000243 Genomic DNA. Translation: ABE07550.1.
RefSeqiYP_541081.1. NC_007946.1.

Genome annotation databases

EnsemblBacteriaiABE07550; ABE07550; UTI89_C2074.
GeneIDi3989667.
KEGGieci:UTI89_C2074.
PATRICi18453724. VBIEscCol42261_2109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000243 Genomic DNA. Translation: ABE07550.1 .
RefSeqi YP_541081.1. NC_007946.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GEK X-ray 1.50 A/G 1-247 [» ]
ProteinModelPortali Q1RAR4.
SMRi Q1RAR4. Positions 20-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 364106.UTI89_C2074.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE07550 ; ABE07550 ; UTI89_C2074 .
GeneIDi 3989667.
KEGGi eci:UTI89_C2074.
PATRICi 18453724. VBIEscCol42261_2109.

Phylogenomic databases

eggNOGi COG0500.
HOGENOMi HOG000218457.
KOi K15256.
OMAi YDLGCSR.
OrthoDBi EOG628F44.

Enzyme and pathway databases

BioCyci ECOL364106:GHPQ-2057-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01589. tRNA_methyltr_CmoA.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view ]
PIRSFi PIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00740. TIGR00740. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
    Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
    Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UTI89 / UPEC.

Entry informationi

Entry nameiCMOA_ECOUT
AccessioniPrimary (citable) accession number: Q1RAR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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