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Q1RAR4

- CMOA_ECOUT

UniProt

Q1RAR4 - CMOA_ECOUT

Protein

tRNA (cmo5U34)-methyltransferase

Gene

cmoA

Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391S-adenosyl-L-methionineUniRule annotation
    Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
    Binding sitei132 – 1321S-adenosyl-L-methionineUniRule annotation
    Binding sitei199 – 1991SubstrateUniRule annotation

    GO - Molecular functioni

    1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA (uracil) methyltransferase activity Source: InterPro

    GO - Biological processi

    1. tRNA wobble uridine modification Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciECOL364106:GHPQ-2057-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (cmo5U34)-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
    Gene namesi
    Name:cmoAUniRule annotation
    Ordered Locus Names:UTI89_C2074
    OrganismiEscherichia coli (strain UTI89 / UPEC)
    Taxonomic identifieri364106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001952: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 247247tRNA (cmo5U34)-methyltransferasePRO_0000314326Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi364106.UTI89_C2074.

    Structurei

    Secondary structure

    1
    247
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi22 – 3514
    Helixi39 – 5315
    Beta strandi59 – 635
    Turni66 – 683
    Helixi69 – 768
    Beta strandi80 – 823
    Beta strandi84 – 907
    Helixi92 – 10312
    Beta strandi111 – 1166
    Turni118 – 1203
    Beta strandi125 – 1339
    Helixi135 – 1373
    Helixi140 – 1423
    Helixi143 – 15311
    Beta strandi154 – 16613
    Helixi171 – 18717
    Helixi190 – 1923
    Helixi196 – 20510
    Helixi211 – 22111
    Beta strandi224 – 2329
    Beta strandi235 – 2417

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GEKX-ray1.50A/G1-247[»]
    ProteinModelPortaliQ1RAR4.
    SMRiQ1RAR4. Positions 20-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 663S-adenosyl-L-methionine bindingUniRule annotation
    Regioni117 – 1182S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. CmoA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0500.
    HOGENOMiHOG000218457.
    KOiK15256.
    OMAiYDLGCSR.
    OrthoDBiEOG628F44.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01589. tRNA_methyltr_CmoA.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR005271. tRNA_cmo5U34_MeTrfase.
    [Graphical view]
    PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1RAR4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA    50
    ERFVQPGTQV YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR 100
    HIDAYKAPTP VDVIEGDIRD IAIENASMVV LNFTLQFLEP SERQALLDKI 150
    YQGLNPGGAL VLSEKFSFED AKVGELLFNM HHDFKRANGY SELEISQKRS 200
    MLENVMLTDS VETHKARLHK AGFEHSELWF QCFNFGSLVA LKAEDAA 247
    Length:247
    Mass (Da):27,791
    Last modified:May 16, 2006 - v1
    Checksum:i2930565165A0A7F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000243 Genomic DNA. Translation: ABE07550.1.
    RefSeqiYP_541081.1. NC_007946.1.

    Genome annotation databases

    EnsemblBacteriaiABE07550; ABE07550; UTI89_C2074.
    GeneIDi3989667.
    KEGGieci:UTI89_C2074.
    PATRICi18453724. VBIEscCol42261_2109.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000243 Genomic DNA. Translation: ABE07550.1 .
    RefSeqi YP_541081.1. NC_007946.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GEK X-ray 1.50 A/G 1-247 [» ]
    ProteinModelPortali Q1RAR4.
    SMRi Q1RAR4. Positions 20-242.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 364106.UTI89_C2074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE07550 ; ABE07550 ; UTI89_C2074 .
    GeneIDi 3989667.
    KEGGi eci:UTI89_C2074.
    PATRICi 18453724. VBIEscCol42261_2109.

    Phylogenomic databases

    eggNOGi COG0500.
    HOGENOMi HOG000218457.
    KOi K15256.
    OMAi YDLGCSR.
    OrthoDBi EOG628F44.

    Enzyme and pathway databases

    BioCyci ECOL364106:GHPQ-2057-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01589. tRNA_methyltr_CmoA.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR005271. tRNA_cmo5U34_MeTrfase.
    [Graphical view ]
    PIRSFi PIRSF006325. MeTrfase_bac. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00740. TIGR00740. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
      Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
      Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: UTI89 / UPEC.

    Entry informationi

    Entry nameiCMOA_ECOUT
    AccessioniPrimary (citable) accession number: Q1RAR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3