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Q1RAR4 (CMOA_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (cmo5U34)-methyltransferase

EC=2.1.1.-
Gene names
Name:cmoA
Ordered Locus Names:UTI89_C2074
OrganismEscherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier364106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity. HAMAP-Rule MF_01589

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01589

Sequence similarities

Belongs to the methyltransferase superfamily. CmoA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247tRNA (cmo5U34)-methyltransferase HAMAP-Rule MF_01589
PRO_0000314326

Regions

Region64 – 663S-adenosyl-L-methionine binding By similarity
Region117 – 1182S-adenosyl-L-methionine binding By similarity

Sites

Binding site391S-adenosyl-L-methionine By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1991Substrate Potential

Secondary structure

........................................ 247
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q1RAR4 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 2930565165A0A7F4

FASTA24727,791
        10         20         30         40         50         60 
MSHRDTLFSA PIARLGDWTF DERVAEVFPD MIQRSVPGYS NIISMIGMLA ERFVQPGTQV 

        70         80         90        100        110        120 
YDLGCSLGAA TLSVRRNIHH DNCKIIAIDN SPAMIERCRR HIDAYKAPTP VDVIEGDIRD 

       130        140        150        160        170        180 
IAIENASMVV LNFTLQFLEP SERQALLDKI YQGLNPGGAL VLSEKFSFED AKVGELLFNM 

       190        200        210        220        230        240 
HHDFKRANGY SELEISQKRS MLENVMLTDS VETHKARLHK AGFEHSELWF QCFNFGSLVA 


LKAEDAA 

« Hide

References

[1]"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UTI89 / UPEC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000243 Genomic DNA. Translation: ABE07550.1.
RefSeqYP_541081.1. NC_007946.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GEKX-ray1.50A/G1-247[»]
ProteinModelPortalQ1RAR4.
SMRQ1RAR4. Positions 20-242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING364106.UTI89_C2074.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE07550; ABE07550; UTI89_C2074.
GeneID3989667.
KEGGeci:UTI89_C2074.
PATRIC18453724. VBIEscCol42261_2109.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0500.
HOGENOMHOG000218457.
KOK15256.
OMADMLERSI.
OrthoDBEOG628F44.
ProtClustDBPRK15451.

Enzyme and pathway databases

BioCycECOL364106:GHPQ-2057-MONOMER.

Family and domain databases

HAMAPMF_01589. tRNA_methyltr_CmoA.
InterProIPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PANTHERPTHR10108:SF119. PTHR10108:SF119. 1 hit.
PIRSFPIRSF006325. MeTrfase_bac. 1 hit.
TIGRFAMsTIGR00740. TIGR00740. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCMOA_ECOUT
AccessionPrimary (citable) accession number: Q1RAR4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references