ID   GATY_ECOUT              Reviewed;         284 AA.
AC   Q1R9X7;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=D-tagatose-1,6-bisphosphate aldolase subunit gatY;
DE            Short=TagBP aldolase;
DE            Short=TBPA;
DE            EC=4.1.2.40;
DE   AltName: Full=Tagatose-bisphosphate aldolase;
DE   AltName: Full=D-tagatose-bisphosphate aldolase class II;
GN   Name=gatY; OrderedLocusNames=UTI89_C2369;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R.,
RA   Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R.,
RA   Hultgren S.J., Gordon J.I.;
RT   "Identification of genes subject to positive selection in
RT   uropathogenic strains of Escherichia coli: a comparative genomics
RT   approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- FUNCTION: Catalytic subunit of the tagatose-1,6-bisphosphate
CC       aldolase gatYZ, which catalyzes the reversible aldol condensation
CC       of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with
CC       glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-
CC       bisphosphate (TBP). Requires gatZ subunit for full activity and
CC       stability. Is involved in the catabolism of galactitol (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: D-tagatose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate
CC       degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate
CC       from D-tagatose 6-phosphate: step 2/2.
CC   -!- SUBUNIT: Forms a complex with gatZ (By similarity).
CC   -!- SIMILARITY: Belongs to the class II fructose-bisphosphate aldolase
CC       family. TagBP aldolase gatY subfamily.
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DR   EMBL; CP000243; ABE07837.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_541368.2; -.
DR   GeneID; 3992117; -.
DR   GenomeReviews; CP000243_GR; UTI89_C2369.
DR   KEGG; eci:UTI89_C2369; -.
DR   HOGENOM; Q1R9X7; -.
DR   BioCyc; ECOL364106:UTI89_C2369-MON; -.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:InterPro.
DR   GO; GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019404; P:galactitol catabolic process; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-ph...; IEA:InterPro.
DR   HAMAP; MF_01294; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000771; Ketose_bisP_aldolase_II.
DR   InterPro; IPR011288; Tag_bisphos_ald.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01116; F_bP_aldolase; 1.
DR   PIRSF; PIRSF001359; F_bP_aldolase_II; 1.
DR   ProDom; PD002376; K_bP_aldolase; 1.
DR   TIGRFAMs; TIGR00167; cbbA; 1.
DR   TIGRFAMs; TIGR01858; tag_bisphos_ald; 1.
DR   PROSITE; PS00602; ALDOLASE_CLASS_II_1; 1.
DR   PROSITE; PS00806; ALDOLASE_CLASS_II_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Galactitol metabolism; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    284       D-tagatose-1,6-bisphosphate aldolase
FT                                subunit gatY.
FT                                /FTId=PRO_0000355341.
FT   REGION      209    211       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   REGION      230    233       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   ACT_SITE     82     82       Proton donor (By similarity).
FT   METAL        83     83       Zinc; catalytic (By similarity).
FT   METAL       180    180       Zinc; catalytic (By similarity).
FT   METAL       208    208       Zinc; catalytic (By similarity).
FT   BINDING     181    181       Dihydroxyacetone phosphate; via amide
FT                                nitrogen (By similarity).
SQ   SEQUENCE   284 AA;  30937 MW;  9C920999A2708E8C CRC64;
     MYVVSTKQML NNARRGGYAV PAFNIHNLET MQVVVETAAS MHAPVIIAGT PGTFTHAGTE
     NLMALVSAMA KQYHHPLAIH LDHHTKFDDI AQKVRSGVRS VMIDASHLPF AQNISRVKEV
     VDFCHRFDVS VEAELGQLGG QEDDVQVNEA DAFYTNPVQA REFAEATGID SLAVAIGTAH
     GMYASAPALD FSRLENIRQW VNLPLVLHGA SGLSTKDIQQ TIKLGICKIN VATELKNAFS
     QALKNYLTEY PEATDPRDYL QSAKSAMRDV VSKVIADCGC EGRA
//