ID   THIM_ECOUT              Reviewed;         262 AA.
AC   Q1R9W8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=Hydroxyethylthiazole kinase;
DE            EC=2.7.1.50;
DE   AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase;
DE            Short=Thz kinase;
DE            Short=TH kinase;
GN   Name=thiM; OrderedLocusNames=UTI89_C2378;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R.,
RA   Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R.,
RA   Hultgren S.J., Gordon J.I.;
RT   "Identification of genes subject to positive selection in
RT   uropathogenic strains of Escherichia coli: a comparative genomics
RT   approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole =
CC       ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate
CC       biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-
CC       5-(2-hydroxyethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the Thz kinase family.
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DR   EMBL; CP000243; ABE07846.1; -; Genomic_DNA.
DR   RefSeq; YP_541377.1; -.
DR   GeneID; 3989648; -.
DR   GenomeReviews; CP000243_GR; UTI89_C2378.
DR   KEGG; eci:UTI89_C2378; -.
DR   HOGENOM; Q1R9W8; -.
DR   OMA; Q1R9W8; ASPVMAH.
DR   BioCyc; ECOL364106:UTI89_C2378-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:HAMAP.
DR   GO; GO:0009228; P:thiamin biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00228; -; 1.
DR   InterPro; IPR000417; Hyethyz_kinase.
DR   InterPro; IPR011144; Hyethyz_kinsmonf.
DR   PANTHER; PTHR20857:SF14; Hyethyz_kinase; 1.
DR   Pfam; PF02110; HK; 1.
DR   PIRSF; PIRSF000513; Thz_kinase; 1.
DR   PRINTS; PR01099; HYETHTZKNASE.
DR   TIGRFAMs; TIGR00694; thiM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Kinase; Nucleotide-binding;
KW   Thiamine biosynthesis; Transferase.
FT   CHAIN         1    262       Hydroxyethylthiazole kinase.
FT                                /FTId=PRO_1000021511.
FT   BINDING      50     50       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     125    125       ATP (By similarity).
FT   BINDING     171    171       ATP (By similarity).
FT   BINDING     198    198       Substrate; via amide nitrogen (By
FT                                similarity).
SQ   SEQUENCE   262 AA;  27311 MW;  5D0B032424415A74 CRC64;
     MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF
     AAIASALLIN VGTLTQPRAQ AMSAAVEQAT RSQTPWTLDP VAVGALDYRR RFCVELLSHK
     PTAIRGNASE IMALAGVANG GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEVDYVTDG
     HRIIGIHGGD PLMTKVVGTG CALSAVVAAC CALPGDTLEN IASACHWMKQ AGERAVARSE
     GPGSFVPHFL DALWQLTQEV QA
//