Q1R9W8 (THIM_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	UTI89_C2378

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021511
Sites
	Binding site	50	1	Substrate; via amide nitrogen By similarity
	Binding site	125	1	ATP By similarity
	Binding site	171	1	ATP By similarity
	Binding site	198	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R9W8 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 5D0B032424415A74	FASTA	262	27,311
	10 20 30 40 50 60 MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF 70 80 90 100 110 120 AAIASALLIN VGTLTQPRAQ AMSAAVEQAT RSQTPWTLDP VAVGALDYRR RFCVELLSHK 130 140 150 160 170 180 PTAIRGNASE IMALAGVANG GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEVDYVTDG 190 200 210 220 230 240 HRIIGIHGGD PLMTKVVGTG CALSAVVAAC CALPGDTLEN IASACHWMKQ AGERAVARSE 250 260 GPGSFVPHFL DALWQLTQEV QA

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE07846.1.
RefSeq	YP_541377.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1R9W8.
SMR	Q1R9W8. Positions 4-261.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1R9W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000070116; EBESCP00000067594; EBESCG00000069163.
GeneID	3989648.
GenomeReviews	Gene locus UTI89_C2378 in contig CP000243_GR.
KEGG	eci:UTI89_C2378.
PATRIC	18454338. VBIEscCol42261_2407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
GeneTree	EBGT00050000010804.
HOGENOM	HBG351126.
OMA	AIRGNAG.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C2378-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_ECOUT

Accession

Primary (citable) accession number: Q1R9W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program