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Q1R972

- FADJ_ECOUT

UniProt

Q1R972 - FADJ_ECOUT

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadJ, UTI89_C2625
Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei118 – 1181Important for catalytic activity By similarity
Sitei140 – 1401Important for catalytic activity By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro
  4. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciECOL364106:GHPQ-2607-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Ordered Locus Names:UTI89_C2625
OrganismiEscherichia coli (strain UTI89 / UPEC)
Taxonomic identifieri364106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001952: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Fatty acid oxidation complex subunit alphaUniRule annotation
PRO_0000273982Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Protein-protein interaction databases

STRINGi364106.UTI89_C2625.

Structurei

3D structure databases

ProteinModelPortaliQ1R972.
SMRiQ1R972. Positions 1-707.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydratase By similarity
Add
BLAST
Regioni306 – 7144093-hydroxyacyl-CoA dehydrogenase By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1R972-1 [UniParc]FASTAAdd to Basket

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MEMASAFTLN VRLDNIAIIT IDVPGEKMNT LKAEFASQVR AIIKQIRENK    50
ELRGVVFVSA KPDNFIAGAD INMIGNCKTA QEAEVLARQG QQLMAEIHAL 100
PIPVIAAIHG ACLGGGLELA LACHGRVCTD DPKTVLGLPE VQLGLLPGSG 150
GTQRLPRLIG VSTALEMILT GKQLRAKQAV KLGLVDDVVP HSILLEAAVE 200
LAKQDRPSSR PLPVRERILA GPLGRALLFK MVGKKTEHKT QGNYPATERI 250
LEVVETGLAQ GTSSGYDAEA RAFGELAMTP QSQALRNIFF ASTDVKKDPG 300
SDAPPAPLNS VGILGGGLMG GGIAYVTACK AGLPVRIKDI NPRGINHALK 350
YSWDQLEGKV RRRHLKASER DKQLALISGT TDYCGFAHRD LIIEAVFENL 400
ELKQQMVAEV EQNCATHTIF ASNTSSLPIG DIAAHAARPE QVIGLHFFSP 450
VEKMPLVEII PHASTSAQTI ATTVKLAKKQ GKTPIVVRDK AGFYVNRILA 500
PYINEAIRML TEGERIEHID AALVKFGFPV GPIQLLDEVG IDTGTKIMPV 550
LEAAYGERFS APANVVSSIL NDDRKGRKNG RGFYLYGQKG RKSKKQVDPA 600
IYPLIGAQGQ GRLSAPQVAE RCVMLMLNEA VRCLDEQVIR SVRDGDIGAV 650
FGIGFPPFLG GPFRYIDSLG AGEVVAIMQR LATQYGSRFT PCDRLVEMSE 700
RGESFWKTTA TDLQ 714
Length:714
Mass (Da):77,148
Last modified:May 16, 2006 - v1
Checksum:iD05212AF62C80111
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000243 Genomic DNA. Translation: ABE08092.1.
RefSeqiYP_541623.1. NC_007946.1.

Genome annotation databases

EnsemblBacteriaiABE08092; ABE08092; UTI89_C2625.
GeneIDi3991795.
KEGGieci:UTI89_C2625.
PATRICi18454828. VBIEscCol42261_2651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000243 Genomic DNA. Translation: ABE08092.1 .
RefSeqi YP_541623.1. NC_007946.1.

3D structure databases

ProteinModelPortali Q1R972.
SMRi Q1R972. Positions 1-707.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 364106.UTI89_C2625.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE08092 ; ABE08092 ; UTI89_C2625 .
GeneIDi 3991795.
KEGGi eci:UTI89_C2625.
PATRICi 18454828. VBIEscCol42261_2651.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci ECOL364106:GHPQ-2607-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
    Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
    Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UTI89 / UPEC.

Entry informationi

Entry nameiFADJ_ECOUT
AccessioniPrimary (citable) accession number: Q1R972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 16, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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