Q1R8X8 (GLK_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucokinase
EC=2.7.1.2

Alternative name(s):
Glucose kinase

Gene names

Name:	glk
Ordered Locus Names:	UTI89_C2720

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. HAMAP-Rule MF_00524
Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP-Rule MF_00524
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the bacterial glucokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Glucokinase HAMAP-Rule MF_00524		PRO_0000268772
Regions
	Nucleotide binding	8 – 13	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1R8X8 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 42ACBF56627A5C98	FASTA	321	34,709
	10 20 30 40 50 60 MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 70 80 90 100 110 120 IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 130 140 150 160 170 180 GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE GIILEILRAE 190 200 210 220 230 240 IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 250 260 270 280 290 300 MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 310 320 HDNPGLLGSG AHLRQTLGHI L

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE08186.1.
RefSeq	YP_541717.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1R8X8.
SMR	Q1R8X8. Positions 2-321.
ModBase	Search...
Protein-protein interaction databases
STRING	364106.UTI89_C2720.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE08186; ABE08186; UTI89_C2720.
GeneID	3989780.
KEGG	eci:UTI89_C2720.
PATRIC	18454994. VBIEscCol42261_2733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0837.
HOGENOM	HOG000274469.
KO	K00845.
OMA	EAVIRHY.
ProtClustDB	PRK00292.
Enzyme and pathway databases
BioCyc	ECOL364106:GHPQ-2768-MONOMER.
Family and domain databases
HAMAP	MF_00524. Glucokinase.
InterPro	IPR003836. Glucokinase. [Graphical view]
Pfam	PF02685. Glucokinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00749. glk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLK_ECOUT

Accession

Primary (citable) accession number: Q1R8X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program