Q1R8V2 (PDXK_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxine kinase
EC=2.7.1.35

Alternative name(s):
PN/PL/PM kinase
Pyridoxal kinase
Pyridoxamine kinase
Vitamin B6 kinase

Gene names

Name:	pdxK
Ordered Locus Names:	UTI89_C2752

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates By similarity. HAMAP MF_01638
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate. HAMAP MF_01638
Cofactor	Zinc or magnesium By similarity. HAMAP MF_01638
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyridoxal 5'-phosphate salvage Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Pyridoxine kinase HAMAP MF_01638		PRO_0000268835
Regions
	Nucleotide binding	195 – 196	2	ATP By similarity
	Nucleotide binding	220 – 232	13	ATP By similarity
Sites
	Binding site	23	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R8V2 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 303C85C8DEDA369D	FASTA	283	30,848
	10 20 30 40 50 60 MSSLLLFNDK SRALQADIVA VQSQVVYGSV GNSIAVPAIK QNGLNVFAVP TVLLSNTPHY 70 80 90 100 110 120 DTFYGGAIPD EWFSGYLRAL QERDALRQLR AVTTGYMGTA SQIKILAEWL TALRKDHPDL 130 140 150 160 170 180 LIMVDPVIGD IDSGIYVKPD LPEAYRQYLL PLAQGITPNI FELEILTGKD CRDLDSAIAA 190 200 210 220 230 240 AKSLLSDTLK WVVITSASGN EENQEMQVVV VSADSVNVIS HSRVKTDLKG TGDLFCAQLI 250 260 270 280 SGLLKGKALT DAVHRAGLRV LEVMRYTQQH ESDELILPPL AEA

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE08212.1.
RefSeq	YP_541743.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1R8V2.
SMR	Q1R8V2. Positions 2-280.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1R8V2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000067261; EBESCP00000064739; EBESCG00000066308.
GeneID	3989917.
GenomeReviews	Gene locus UTI89_C2752 in contig CP000243_GR.
KEGG	eci:UTI89_C2752.
PATRIC	18455056. VBIEscCol42261_2758.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2240.
GeneTree	EBGT00050000009567.
HOGENOM	HBG661459.
OMA	TRDASFL.
ProtClustDB	PRK08176.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C2752-MONOMER.
Family and domain databases
HAMAP	MF_01638. PdxK. [Tree]
InterPro	IPR013749. HMP-P_kinase-1. IPR004625. PyrdxlP_synth_PyrdxlKinase. IPR023479. Pyridoxal_kinase_PdxK. [Graphical view]
KO	K00868.
PANTHER	PTHR10534. PTHR10534. 1 hit.
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00687. Pyridox_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXK_ECOUT

Accession

Primary (citable) accession number: Q1R8V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program