Q1R8H7 (PUR4_ECOUT) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 49.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phosphoribosylformylglycinamidine synthase Short name=FGAM synthase Short name=FGAMS EC=6.3.5.3 Alternative name(s): Formylglycinamide ribotide amidotransferase Short name=FGARAT Formylglycinamide ribotide synthetase | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 364106 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 1295 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419 |
| Pathway | Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419 |
| Subunit structure | Monomer. |
| Subcellular location | |
| Sequence similarities | In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain. |
| Sequence caution | The sequence ABE08337.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Cellular component | Cytoplasm |
| Domain | Glutamine amidotransferase |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | 'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1295 | 1295 | Phosphoribosylformylglycinamidine synthase HAMAP MF_00419 | PRO_0000264573 | |||||
Regions | |||||||||
| Domain | 1042 – 1295 | 254 | Glutamine amidotransferase type-1 | ||||||
| Nucleotide binding | 307 – 318 | 12 | ATP Potential | ||||||
Sites | |||||||||
| Active site | 1135 | 1 | Nucleophile By similarity | ||||||
| Active site | 1260 | 1 | By similarity | ||||||
| Active site | 1262 | 1 | By similarity | ||||||
Sequences
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References
| [1] | "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000243 Genomic DNA. Translation: ABE08337.1. Different initiation. |
| RefSeq | YP_541868.1. NC_007946.1. |
3D structure databases | |
| ProteinModelPortal | Q1R8H7. |
| SMR | Q1R8H7. Positions 1039-1294. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q1R8H7. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000069627; EBESCP00000067105; EBESCG00000068674. |
| GeneID | 3989788. |
| GenomeReviews | Gene locus UTI89_C2877 in contig CP000243_GR. |
| KEGG | eci:UTI89_C2877. |
| PATRIC | 18455294. VBIEscCol42261_2877. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0046. |
| GeneTree | EBGT00050000010521. |
| HOGENOM | HBG335309. |
| OMA | VKAVGME. |
| ProtClustDB | PRK05297. |
Enzyme and pathway databases | |
| BioCyc | ECOL364106:UTI89_C2877-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00419. PurL_1. [Tree] |
| InterPro | IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR022940. PRibForGlyAmidine_synth_bac. IPR016188. PurM_N-like. [Graphical view] |
| KO | K01952. |
| Pfam | PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view] |
| SUPFAM | SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits. |
| TIGRFAMs | TIGR01735. FGAM_synt. 1 hit. |
| PROSITE | PS51273. GATASE_TYPE_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR4_ECOUT | ||||||||
| Accession | Primary (citable) accession number: Q1R8H7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |