Q1R7U1 (CYSC_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylyl-sulfate kinase
EC=2.7.1.25

Alternative name(s):
APS kinase
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase

Gene names

Name:	cysC
Ordered Locus Names:	UTI89_C3121

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate. HAMAP MF_00065
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate. HAMAP MF_00065
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. HAMAP MF_00065
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylylsulfate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Adenylyl-sulfate kinase HAMAP MF_00065		PRO_1000009012
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity
Sites
	Active site	109	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R7U1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 5F474514DCA61FBB	FASTA	201	22,334
	10 20 30 40 50 60 MALHDENVVW HSHPVTPQQR EQHHGHRGVV LWFTGLSGSG KSTVAGALEE ALHKLGVSTY 70 80 90 100 110 120 LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG LVVLTAFISP HRAERQMVRE 130 140 150 160 170 180 RVGEGRFIEV FVDTPLAICE ARDPKGLYKK ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ 190 200 LVTNLVQQLL DLLRQNDIIR S

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE08573.1.
RefSeq	YP_542104.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1R7U1.
SMR	Q1R7U1. Positions 14-200.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1R7U1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000068655; EBESCP00000066133; EBESCG00000067702.
GeneID	3990446.
GenomeReviews	Gene locus UTI89_C3121 in contig CP000243_GR.
KEGG	eci:UTI89_C3121.
PATRIC	18455762. VBIEscCol42261_3103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0529.
GeneTree	EBGT00050000011347.
HOGENOM	HBG309946.
OMA	AELGYHT.
ProtClustDB	PRK03846.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C3121-MONOMER.
Family and domain databases
HAMAP	MF_00065. Adenylyl_sulf_kinase. [Tree]
InterPro	IPR002891. APS_kinase_C. [Graphical view]
KO	K00860.
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00455. ApsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSC_ECOUT

Accession

Primary (citable) accession number: Q1R7U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program