Reviewed, UniProtKB/Swiss-Prot Q1R7N8 (FUCI_ECOUT)

Names and origin

Protein names

Recommended name:
    L-fucose isomerase
    EC=5.3.1.25
Alternative name(s):
    6-deoxy-L-galactose isomerase
    FucIase

Gene names

Name:	fucI
Ordered Locus Names:	UTI89_C3174

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts the aldose L-fucose into the corresponding ketose L-fuculose By similarity.
Catalytic activity	L-fucose = L-fuculose. HAMAP MF_01254
Cofactor	Manganese By similarity.
Pathway	Carbohydrate degradation; L-fucose degradation; L-lactaldehyde and glycerone phosphate from L-fucose: step 1/3. HAMAP MF_01254
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-fucose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fucose catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-fucose isomerase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	L-fucose isomerase HAMAP MF_01254		PRO_1000067220
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	361	1	Proton acceptor By similarity
	Metal binding	337	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	528	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R7N8-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: E1519E4EDA35128E	FASTA	591	64,990
	10 20 30 40 50 60 MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL LTEKLRHACG AAVECVISDT 70 80 90 100 110 120 CIAGMAEAAA CEEKFSSQNV GLTITVTPCW CYGSETIDMD PTRPKAIWGF NGTERPGAVY 130 140 150 160 170 180 LAAALAAHSQ KGIPAFSIYG HDVQDADDTS IPADVEEKLL RFARAGLAVA SMKGKSYLSL 190 200 210 220 230 240 GGVSMGIAGS IVDHNFFESW LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY 250 260 270 280 290 300 GEDENNKQYQ RNAEQSRAVL RESLLMAMCI RDMMQGNSKL ADIGRVEESL GYNAIAAGFQ 310 320 330 340 350 360 GQRHWTDQYP NGDTAEAILN SSFDWNGVRK PFVVATENDS LNGVAMLMGH QLTGTAQVFA 370 380 390 400 410 420 DVRTYWSPEA IERVTGHKLD GLAEHGIIHL INSGSAALDG SCKQRDSEGK PTMKPHWEIS 430 440 450 460 470 480 QQEADACLAA TEWCPAIHEY FRGGGYSSRF LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS 490 500 510 520 530 540 VELPKDVHDI LNKRTNSTWP TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF 550 560 570 580 590 ITLASMLRIP VCMHNVEETK VYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000243 Genomic DNA. Translation: ABE08626.1.
RefSeq	YP_542157.1.
3D structure databases
SMR	Q1R7N8. Positions 1-591.
ModBase	Search...
Genome annotation databases
GeneID	3990309.
GenomeReviews	Gene locus UTI89_C3174 in contig CP000243_GR.
KEGG	eci:UTI89_C3174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1R7N8.
OMA	Q1R7N8. TWFVPRL.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C3174-MON.
Family and domain databases
HAMAP	MF_01254. [Tree]
InterPro	IPR015888. Fuc_isomerase-like_C. IPR004216. Fuc_isomerase_C. IPR012888. Fucose_iso_N1. IPR005763. Fucose_isomerase. IPR012889. Fucose_isomerase_N2. [Graphical view]
Gene3D	G3DSA:3.20.14.10. Fuc_isomerase_C. 1 hit. G3DSA:3.40.50.1070. Fucose_iso_N1. 1 hit. G3DSA:3.40.275.10. Fucose_iso_N2. 1 hit.
Pfam	PF02952. Fucose_iso_C. 1 hit. PF07881. Fucose_iso_N1. 1 hit. PF07882. Fucose_iso_N2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01089. fucI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FUCI_ECOUT

Accession

Primary (citable) accession number: Q1R7N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)