Q1R6L1 (GARL_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-keto-4-deoxy-D-glucarate aldolase
Short name=KDGluc aldolase
Short name=KDGlucA
EC=4.1.2.20

Alternative name(s):
2-dehydro-3-deoxy-D-glucarate aldolase
2-keto-3-deoxy-D-glucarate aldolase
5-dehydro-4-deoxy-D-glucarate aldolase
Alpha-keto-beta-deoxy-D-glucarate aldolase

Gene names

Name:	garL
Ordered Locus Names:	UTI89_C3557

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde By similarity. HAMAP MF_01291
Catalytic activity	5-dehydro-4-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01291
Pathway	Carbohydrate acid metabolism; D-galactarate degradation; D-glycerate from D-galactarate: step 2/3. HAMAP MF_01291
Subunit structure	Homohexamer; trimer of dimers By similarity. HAMAP MF_01291
Sequence similarities	Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-dehydro-3-deoxyglucarate aldolase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	5-keto-4-deoxy-D-glucarate aldolase HAMAP MF_01291		PRO_0000353145
Sites
	Active site	50	1	Proton acceptor By similarity
	Metal binding	153	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	178	1	Substrate; via amide nitrogen By similarity
	Binding site	179	1	Substrate By similarity
	Site	75	1	Transition state stabilizer By similarity
	Site	89	1	Increases basicity of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R6L1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: A363593FFB171401	FASTA	256	27,399
	10 20 30 40 50 60 MNNDVFPNKF KAALAAKQVQ IGCWSALSNP ISTEVLGLAG FDWLVLDGEH APNDISTFIP 70 80 90 100 110 120 QLMALKGSAS APVVRVPTNE PVIIKRLLDI GFYNFLIPFV ETKEEAEQAV ASTRYPPEGI 130 140 150 160 170 180 RGVSVSHRAN MFGTVADYFA QSNKNITILV QIESQQGVDN VDAIAATEGV DGIFVGPSDL 190 200 210 220 230 240 AAALGHLGNA SHPDVQKAIQ HIFNRASAHG KPSGILAPVE ADARRYLEWG ATFVAVGSDL 250 GVFRSATQKL ADTFKK

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000243 Genomic DNA. Translation: ABE09003.1.
RefSeq	YP_542534.1. NC_007946.1.
3D structure databases
ProteinModelPortal	Q1R6L1.
SMR	Q1R6L1. Positions 4-256.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1R6L1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000068844; EBESCP00000066322; EBESCG00000067891.
GeneID	3990328.
GenomeReviews	Gene locus UTI89_C3557 in contig CP000243_GR.
KEGG	eci:UTI89_C3557.
PATRIC	18456614. VBIEscCol42261_3523.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3836.
GeneTree	EBGT00050000008793.
HOGENOM	HBG747616.
OMA	IGCWCAL.
ProtClustDB	PRK10558.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C3557-MONOMER.
Family and domain databases
HAMAP	MF_01291. KDGluc_aldolase. [Tree]
InterPro	IPR005000. Aldehyde-lyase_domain. IPR017648. Dehyd-dGlucarate-aldolase_GarL. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K01630.
Pfam	PF03328. HpcH_HpaI. 1 hit. [Graphical view]
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR03239. GarL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GARL_ECOUT

Accession

Primary (citable) accession number: Q1R6L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program