ID   KBAY_ECOUT              Reviewed;         286 AA.
AC   Q1R6K0;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=D-tagatose-1,6-bisphosphate aldolase subunit kbaY;
DE            Short=TagBP aldolase;
DE            Short=TBPA;
DE            EC=4.1.2.40;
DE   AltName: Full=Tagatose-bisphosphate aldolase;
DE   AltName: Full=D-tagatose-bisphosphate aldolase class II;
DE   AltName: Full=Ketose 1,6-bisphosphate aldolase class II;
GN   Name=kbaY; OrderedLocusNames=UTI89_C3568;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R.,
RA   Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R.,
RA   Hultgren S.J., Gordon J.I.;
RT   "Identification of genes subject to positive selection in
RT   uropathogenic strains of Escherichia coli: a comparative genomics
RT   approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- FUNCTION: Catalytic subunit of the tagatose-1,6-bisphosphate
CC       aldolase kbaYZ, which catalyzes the reversible aldol condensation
CC       of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with
CC       glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-
CC       bisphosphate (TBP). Requires kbaZ subunit for full activity and
CC       stability (By similarity).
CC   -!- CATALYTIC ACTIVITY: D-tagatose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate
CC       degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate
CC       from D-tagatose 6-phosphate: step 2/2.
CC   -!- SUBUNIT: Homotetramer. Forms a complex with kbaZ (By similarity).
CC   -!- SIMILARITY: Belongs to the class II fructose-bisphosphate aldolase
CC       family. TagBP aldolase kbaY subfamily.
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DR   EMBL; CP000243; ABE09014.1; -; Genomic_DNA.
DR   RefSeq; YP_542545.1; -.
DR   SMR; Q1R6K0; 2-284.
DR   GeneID; 3992479; -.
DR   GenomeReviews; CP000243_GR; UTI89_C3568.
DR   KEGG; eci:UTI89_C3568; -.
DR   HOGENOM; Q1R6K0; -.
DR   OMA; Q1R6K0; TIELGVC.
DR   BioCyc; ECOL364106:UTI89_C3568-MON; -.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:InterPro.
DR   GO; GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-ph...; IEA:InterPro.
DR   HAMAP; MF_01293; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000771; Ketose_bisP_aldolase_II.
DR   InterPro; IPR011288; Tag_bisphos_ald.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01116; F_bP_aldolase; 1.
DR   PIRSF; PIRSF001359; F_bP_aldolase_II; 1.
DR   ProDom; PD002376; K_bP_aldolase; 1.
DR   TIGRFAMs; TIGR00167; cbbA; 1.
DR   TIGRFAMs; TIGR01858; tag_bisphos_ald; 1.
DR   PROSITE; PS00602; ALDOLASE_CLASS_II_1; 1.
DR   PROSITE; PS00806; ALDOLASE_CLASS_II_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    286       D-tagatose-1,6-bisphosphate aldolase
FT                                subunit kbaY.
FT                                /FTId=PRO_0000355324.
FT   REGION      209    211       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   REGION      230    233       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   ACT_SITE     82     82       Proton donor (By similarity).
FT   METAL        83     83       Zinc; catalytic (By similarity).
FT   METAL       180    180       Zinc; catalytic (By similarity).
FT   METAL       208    208       Zinc; catalytic (By similarity).
FT   BINDING     181    181       Dihydroxyacetone phosphate; via amide
FT                                nitrogen (By similarity).
SQ   SEQUENCE   286 AA;  31294 MW;  CAA42DF05C2B918B CRC64;
     MSIISTKYLL QDAQANGYAV PAFNIHNAET IQAILEVCSE MRSPVILAGT PGTFKHIALE
     EIYALCSAYS TTYNMPLALH LDHHESLDDI RRKVHAGVRS AMIDGSHFPF AENVKLVKSV
     VDFCHSQDCS VEAELGRLGG VEDDMSVDAE SAFLTDPQEA KRFVELTGVD SLAVAIGTAH
     GLYSKTPKID FQRLAEIREV VDVPLVLHGA SDVPDEFVRR TIELGVTKVN VATELKIAFA
     GAVKAWFAEN PQGNDPRYYM RVGMDAMKEV VRNKINVCGS ANRISA
//