Reviewed, UniProtKB/Swiss-Prot Q1R536 (SYGA_ECOUT)

Names and origin

Protein names

Recommended name:
    Glycyl-tRNA synthetase alpha subunit
    EC=6.1.1.14
Alternative name(s):
    Glycine--tRNA ligase alpha subunit
      Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	UTI89_C4100

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00254
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Glycyl-tRNA synthetase alpha subunit HAMAP MF_00254		PRO_1000047420

Sequences

	Sequence		Length	Mass (Da)
	Q1R536-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 8C22062079FD4DDA	FASTA	303	34,716
	10 20 30 40 50 60 MQKFDTRTFQ GLILTLQDYW ARQGCTIVQP LDMEVGAGTS HPMTCLRALG PEPMAAAYVQ 70 80 90 100 110 120 PSRRPTDGRY GENPNRLQHY YQFQVVIKPS PDNIQELYLG SLKELGMDPT IHDIRFVEDN 130 140 150 160 170 180 WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLECKPVT GEITYGLERL AMYIQGVDSV 190 200 210 220 230 240 YDLVWSDGPL GKTTYGDVFH QNEVEQSTYN FEYADVDFLF TCFEQYEKEA QQLLALENPL 250 260 270 280 290 300 PLPAYERILK AAHSFNLLDA RKAISVTERQ RYILRIRTLT KAVAEAYYAS REALGFPMCN KDK

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed: 16585510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000243 Genomic DNA. Translation: ABE09528.1.
RefSeq	YP_543059.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3991273.
GenomeReviews	Gene locus UTI89_C4100 in contig CP000243_GR.
KEGG	eci:UTI89_C4100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1R536.
OMA	Q1R536. CRRPTDG.
Enzyme and pathway databases
BioCyc	ECOL364106:UTI89_C4100-MON.
Family and domain databases
HAMAP	MF_00254. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
ProDom	PD006985. tRNA_synt_2e. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_ECOUT

Accession

Primary (citable) accession number: Q1R536

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)