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Protein

Nucleoid occlusion factor SlmA

Gene

ttk

Organism
Escherichia coli (strain UTI89 / UPEC)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.UniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi47 – 6620H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. negative regulation of barrier septum assembly Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciECOL364106:GHPQ-4147-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoid occlusion factor SlmAUniRule annotationSAAS annotation
Gene namesi
Name:ttkImported
Synonyms:slmAUniRule annotation
Ordered Locus Names:UTI89_C4185Imported
OrganismiEscherichia coli (strain UTI89 / UPEC)Imported
Taxonomic identifieri364106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001952: Chromosome

Subcellular locationi

Cytoplasmnucleoid UniRule annotationSAAS annotation

GO - Cellular componenti

  1. bacterial nucleoid Source: UniProtKB-HAMAP
  2. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer. Interacts with FtsZ.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi364106.UTI89_C4185.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GCLX-ray2.65A/B/C/D/E/F/G/H1-212[»]
ProteinModelPortaliQ1R4V1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 8461HTH tetR-typeUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili131 – 15828UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nucleoid occlusion factor SlmA family.UniRule annotation
Contains 1 HTH tetR-type DNA-binding domain.UniRule annotation

Keywords - Domaini

Coiled coilUniRule annotation

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1R4V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPGKCLFSG VFCNMAEKQT AKRNRREEIL QSLALMLESS DGSQRITTAK
60 70 80 90 100
LAASVGVSEA ALYRHFPSKT RMFDSLIEFI EDSLITRINL ILKDEKDTTA
110 120 130 140 150
RLRLIVLLLL GFGERNPGLT RILTGHALMF EQDRLQGRIN QLFERIEAQL
160 170 180 190 200
RQVLREKRMR EGEGYATDET LLASQILAFC EGMLSRFVRS EFKYRPTDDF
210
DARWPLIAAQ LQ
Length:212
Mass (Da):24,288
Last modified:May 16, 2006 - v1
Checksum:i378A696615488F38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000243 Genomic DNA. Translation: ABE09613.1.
RefSeqiYP_543144.2. NC_007946.1.

Genome annotation databases

EnsemblBacteriaiABE09613; ABE09613; UTI89_C4185.
GeneIDi3991319.
KEGGieci:UTI89_C4185.
PATRICi18457790. VBIEscCol42261_4092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000243 Genomic DNA. Translation: ABE09613.1.
RefSeqiYP_543144.2. NC_007946.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GCLX-ray2.65A/B/C/D/E/F/G/H1-212[»]
ProteinModelPortaliQ1R4V1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi364106.UTI89_C4185.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE09613; ABE09613; UTI89_C4185.
GeneIDi3991319.
KEGGieci:UTI89_C4185.
PATRICi18457790. VBIEscCol42261_4092.

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.

Enzyme and pathway databases

BioCyciECOL364106:GHPQ-4147-MONOMER.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach."
    Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I.
    Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UTI89 / UPECImported.
  2. "SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid."
    Tonthat N.K., Milam S.L., Chinnam N., Whitfill T., Margolin W., Schumacher M.A.
    Proc. Natl. Acad. Sci. U.S.A. 110:10586-10591(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).

Entry informationi

Entry nameiQ1R4V1_ECOUT
AccessioniPrimary (citable) accession number: Q1R4V1
Entry historyi
Integrated into UniProtKB/TrEMBL: May 16, 2006
Last sequence update: May 16, 2006
Last modified: March 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.