Q1R3B6 (CH60_ECOUT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60 kDa chaperonin

Alternative name(s):
GroEL protein
Protein Cpn60

Gene names

Name:	groL
Synonyms:	groEL
Ordered Locus Names:	UTI89_C4741

Organism

Escherichia coli (strain UTI89 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

364106 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity. HAMAP-Rule MF_00600
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	protein refolding Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	60 kDa chaperonin HAMAP-Rule MF_00600		PRO_0000256909
Amino acid modifications
	Modified residue	117	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R3B6 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: CD3A0FB505F74AD1	FASTA	548	57,329
	10 20 30 40 50 60 MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI 70 80 90 100 110 120 ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI 130 140 150 160 170 180 DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG 190 200 210 220 230 240 TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV 250 260 270 280 290 300 AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV 310 320 330 340 350 360 ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY 370 380 390 400 410 420 DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI 430 440 450 460 470 480 RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA 490 500 510 520 530 540 ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM

References

[1]

"Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach." Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A., Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S., Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTI89 / UPEC.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000243 Genomic DNA. Translation: ABE10148.1.

RefSeq

YP_543679.1. NC_007946.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YNJ	electron microscopy	8.40	A/B/C/D/E/F/G/H/I/J/K/L/M/N	2-525	[»]
3E76	X-ray	3.94	A/B/C/D/E/F/G/H/I/J/K/L/M/N	2-548	[»]

ProteinModelPortal

Q1R3B6.

SMR

Q1R3B6. Positions 2-525.

ModBase

Search...

Protein-protein interaction databases

STRING

364106.UTI89_C4741.

Proteomic databases

PRIDE

Q1R3B6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ABE10148; ABE10148; UTI89_C4741.

GeneID

3991044.

KEGG

eci:UTI89_C4741.

PATRIC

18458884. VBIEscCol42261_4618.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0459.

HOGENOM

HOG000076290.

KO

K04077.

OMA

YNGVITV.

ProtClustDB

PRK00013.

Enzyme and pathway databases

BioCyc

ECOL364106:GHPQ-4701-MONOMER.

Family and domain databases

HAMAP

MF_00600. CH60.

InterPro

IPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
[Graphical view]

PANTHER

PTHR11353. PTHR11353. 1 hit.

Pfam

PF00118. Cpn60_TCP1. 1 hit.
[Graphical view]

PRINTS

PR00298. CHAPERONIN60.

SUPFAM

SSF48592. GroEL-ATPase. 1 hit.

TIGRFAMs

TIGR02348. GroEL. 1 hit.

PROSITE

PS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q1R3B6.

Entry information

Entry name

CH60_ECOUT

Accession

Primary (citable) accession number: Q1R3B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program