Q1R185 (KATG_CHRSD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Csal_0159

Organism

Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]

Taxonomic identifier

290398 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	Catalase-peroxidase HAMAP MF_01961		PRO_0000354761
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1R185 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 38EB720EB9D80F35	FASTA	717	80,005
	10 20 30 40 50 60 MSGKCPVMHG GNTSTGTSNK DWWPEGINLD ILHQHDRKTN PMDPDFNYRE EVKKLDVQAL 70 80 90 100 110 120 KNDLRQLMVD SQAWWPADWG SYVGMFARVA WHSAGSYRLA DGRGGGGTGN QRFAPLNSWP 130 140 150 160 170 180 DNVNTDKGRR LLWPIKKKYG NKISWADLMV LSGTIAYEVA GLKTYGFAFG REDIWHPEKD 190 200 210 220 230 240 IYWGDEKEWL APSDERYADV EKPDTMENPL AAVQMGLIYV NPEGVNGQPD PQKTAEQVRE 250 260 270 280 290 300 TFARMAMNDE ETAALTAGGH TIGKCHGNGE AENLSAEPEA ADVEYQGIGW MNTKGRGIGR 310 320 330 340 350 360 DTVVSGIEGA WTKNPTQWDM GWFDMLFNHE WELKKSPAGA WQWEPVDIAE EDMPVDVEDP 370 380 390 400 410 420 SIRRMPIMTD ADMAMKVDPV YNEICRKFMD DPEYFSETFA KAWFKLTHRD LGPKARYIGP 430 440 450 460 470 480 DVPADDLIWQ DPIPAGSIAY CEEVVKQKIA ESGLSIGEMV STAWDSARTY RGSDMRGGAN 490 500 510 520 530 540 GARIRLAPQK TWPGNEPERL AKVLDVYERI SAETGASIAD VIVLGGSVGI ERAAKAAGHD 550 560 570 580 590 600 VHVPFLKGRG DATDEMTDAA SFAPLEPLAD GFRNWQKQDY VVKPEEMLLD RAQLMGLTGP 610 620 630 640 650 660 EMTVLIGGMR VLGTNHGGTR HGVFTDREGQ LTNDFFVNLT DMGNTWKPAG NNVYEIRDRE 670 680 690 700 710 TDAVKWTASR VDLVFGSNSL LRSYAEVYAQ DDNEEKFVND FVAAWTKVMN ADRFDVA

References

[1]

"Complete sequence of Chromohalobacter salexigens DSM 3043." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Saunders E., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Csonka L.N., O'Conner K., Vreeland R.H., Oren A., Vargas C., Nieto J., Arahal D.R., Goodner B., Wheeler C., Hall P., Ewing A., Benson L., McBeath D., Canovas D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000285 Genomic DNA. Translation: ABE57523.1.
RefSeq	YP_572222.1. NC_007963.1.
3D structure databases
ProteinModelPortal	Q1R185.
SMR	Q1R185. Positions 18-716.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1R185.
Protein family/group databases
PeroxiBase	2697. CsalCP01_DSM3043.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4026228.
GenomeReviews	Gene locus Csal_0159 in contig CP000285_GR.
KEGG	csa:Csal_0159.
NMPDR	fig|290398.4.peg.579.
PATRIC	21444008. VBIChrSal113723_0163.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	CSAL290398:CSAL_0159-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_CHRSD

Accession

Primary (citable) accession number: Q1R185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program