Q1R0J2 (LLDD_CHRSD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	Csal_0404

Organism

Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]

Taxonomic identifier

290398 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter › Chromohalobacter salexigens

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP-Rule MF_01559
Cofactor	FMN By similarity. HAMAP-Rule MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lactate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	L-lactate dehydrogenase [cytochrome] HAMAP-Rule MF_01559		PRO_1000068977
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1R0J2 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 631A171A271F1091	FASTA	392	42,414
	10 20 30 40 50 60 MIISASTDYR HAAKRRLPPF LFHYADGGAY AEHTLRRNVE DLAGIALRQR VLKDMSHLSL 70 80 90 100 110 120 ETELFGEPLA MPVALAPVGL AGMYARRGEV QAARAAASKG IPFTLSTVSV CPIAEVASAI 130 140 150 160 170 180 ERPLWFQLYV LRDRGFMKHV LERAKAAGVK TLVFTVDMPV PGARYRDAHS GMSGKHGGLR 190 200 210 220 230 240 RMLQAVTHPS WAWDVGLHGR PHDLGNVSDY RGQPTELEDY IAWLGNNFDP SISWKDLEWI 250 260 270 280 290 300 REFWDGPMII KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVPSTARAL PAIADAVKGD 310 320 330 340 350 360 LAILADSGVR NGLDVVRMIA MGADTILLGR AYIYALATAG EAGVAHLLEL FEKEMRVAMT 370 380 390 LTGARSIAEL GSDSLVTGSA AASIERTLSP SL

References

[1]

"Complete sequence of Chromohalobacter salexigens DSM 3043." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Saunders E., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Csonka L.N., O'Conner K., Vreeland R.H., Oren A., Vargas C., Nieto J., Arahal D.R., Goodner B., Wheeler C., Hall P., Ewing A., Benson L., McBeath D., Canovas D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000285 Genomic DNA. Translation: ABE57766.1.
RefSeq	YP_572465.1. NC_007963.1.
3D structure databases
ProteinModelPortal	Q1R0J2.
ModBase	Search...
Protein-protein interaction databases
STRING	290398.Csal_0404.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE57766; ABE57766; Csal_0404.
GeneID	4025998.
KEGG	csa:Csal_0404.
PATRIC	21444518. VBIChrSal113723_0418.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HOG000217464.
KO	K00101.
OMA	EGPEMSL.
ProtClustDB	PRK11197.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01559. L-lact_dehydr.
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_CHRSD

Accession

Primary (citable) accession number: Q1R0J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program