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Q1R0B5

- SYI_CHRSD

UniProt

Q1R0B5 - SYI_CHRSD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Csal_0481
    OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
    Taxonomic identifieri290398 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
    ProteomesiUP000000239: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 946946Isoleucine--tRNA ligasePRO_1000022057Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290398.Csal_0481.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1R0B5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1R0B5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKHTLNL PETDFPMRGM LPKREPGRVS QWQDMNLYQR LRDARAGREV    50
    FVLHDGPPYA NGSIHIGHAV NKILKDIVVK SKSLAGYDAP YVPGWDCHGL 100
    PIEHKVETTH GKHLEADEAR GLCREYAGAQ IEGQKTDFVR LGVVGDWENP 150
    YRTMDFANEA GEIRALAKMV EGGYVFKGLK PVNWCFDCGS ALAEAEVEYA 200
    DKKSDAIDVA FAAAEPEALA SAFGLASLDK PAAIVIWTTT PWTIPANQAL 250
    NVHPDFTYAL VDTGELLLVL AEELVESCLT RYGLEGDVVA TAAGASLEFI 300
    TFRHPFYERV APVYLADYVT VEEGSTGIVH SAPSYGLDDF MTCREHGMSF 350
    DDMLNPVQGN GVYRDDLPLF GGQMIWKANP HIVDTLREVN ALMAHTPITH 400
    SYMHCWRHKT PVIYRATAQW FVGMDREDDQ GRTLRQRALD GVEATQFIPA 450
    WGKARLHSMI ANRPDWCISR QRNWGVPIPF FMHKQTGEWH PRTVELMEDV 500
    ARRVEAEGID AWFRLDAREL LGDEADQYDK VTDTLDVWFD SGTTHRHVMR 550
    GSHPMGHDQG PRADLYLEGS DQHRGWFHSS LLTGCAIDGH APYKALLTHG 600
    FTVDEKGRKM SKSMGNVIAP QQVMDKLGAD ILRLWVASTD YSGEMAVSDE 650
    ILKRTADVYR RIRNTSRFLL ANLNGFAPER DAVAFEDMLA LDQWVVDRAA 700
    QLQARIQGAY AEYRFRDVYQ QVHDFCAHEL GGFYLDVIKD RQYTTQADSR 750
    SRRSCQTALY HVVEALVRWV APILSFTAEE IYEVIPGTRG DSVLLEEYYP 800
    HLATLEANAA MGREFWSRVL TVKQAVNKCL EDARNAKVVR NSLAAEVTLY 850
    ADDSLRETLT QLGEELRFVL LTSEVHLASL EDAGSAQAEA SELEGLKVAV 900
    VSSPHAKCER CWHHRADVGT HAADPNLCGR CLSNLPEGPG ETRRYA 946
    Length:946
    Mass (Da):106,075
    Last modified:May 16, 2006 - v1
    Checksum:i406F144C22F5787F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000285 Genomic DNA. Translation: ABE57843.1.
    RefSeqiYP_572542.1. NC_007963.1.

    Genome annotation databases

    EnsemblBacteriaiABE57843; ABE57843; Csal_0481.
    GeneIDi4026505.
    KEGGicsa:Csal_0481.
    PATRICi21444694. VBIChrSal113723_0496.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000285 Genomic DNA. Translation: ABE57843.1 .
    RefSeqi YP_572542.1. NC_007963.1.

    3D structure databases

    ProteinModelPortali Q1R0B5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290398.Csal_0481.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE57843 ; ABE57843 ; Csal_0481 .
    GeneIDi 4026505.
    KEGGi csa:Csal_0481.
    PATRICi 21444694. VBIChrSal113723_0496.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

    Entry informationi

    Entry nameiSYI_CHRSD
    AccessioniPrimary (citable) accession number: Q1R0B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3