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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Csal_0481
OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifieri290398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
ProteomesiUP000000239 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Isoleucine--tRNA ligasePRO_1000022057Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290398.Csal_0481.

Structurei

3D structure databases

ProteinModelPortaliQ1R0B5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1R0B5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETDFPMRGM LPKREPGRVS QWQDMNLYQR LRDARAGREV
60 70 80 90 100
FVLHDGPPYA NGSIHIGHAV NKILKDIVVK SKSLAGYDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVETTH GKHLEADEAR GLCREYAGAQ IEGQKTDFVR LGVVGDWENP
160 170 180 190 200
YRTMDFANEA GEIRALAKMV EGGYVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKKSDAIDVA FAAAEPEALA SAFGLASLDK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPDFTYAL VDTGELLLVL AEELVESCLT RYGLEGDVVA TAAGASLEFI
310 320 330 340 350
TFRHPFYERV APVYLADYVT VEEGSTGIVH SAPSYGLDDF MTCREHGMSF
360 370 380 390 400
DDMLNPVQGN GVYRDDLPLF GGQMIWKANP HIVDTLREVN ALMAHTPITH
410 420 430 440 450
SYMHCWRHKT PVIYRATAQW FVGMDREDDQ GRTLRQRALD GVEATQFIPA
460 470 480 490 500
WGKARLHSMI ANRPDWCISR QRNWGVPIPF FMHKQTGEWH PRTVELMEDV
510 520 530 540 550
ARRVEAEGID AWFRLDAREL LGDEADQYDK VTDTLDVWFD SGTTHRHVMR
560 570 580 590 600
GSHPMGHDQG PRADLYLEGS DQHRGWFHSS LLTGCAIDGH APYKALLTHG
610 620 630 640 650
FTVDEKGRKM SKSMGNVIAP QQVMDKLGAD ILRLWVASTD YSGEMAVSDE
660 670 680 690 700
ILKRTADVYR RIRNTSRFLL ANLNGFAPER DAVAFEDMLA LDQWVVDRAA
710 720 730 740 750
QLQARIQGAY AEYRFRDVYQ QVHDFCAHEL GGFYLDVIKD RQYTTQADSR
760 770 780 790 800
SRRSCQTALY HVVEALVRWV APILSFTAEE IYEVIPGTRG DSVLLEEYYP
810 820 830 840 850
HLATLEANAA MGREFWSRVL TVKQAVNKCL EDARNAKVVR NSLAAEVTLY
860 870 880 890 900
ADDSLRETLT QLGEELRFVL LTSEVHLASL EDAGSAQAEA SELEGLKVAV
910 920 930 940
VSSPHAKCER CWHHRADVGT HAADPNLCGR CLSNLPEGPG ETRRYA
Length:946
Mass (Da):106,075
Last modified:May 16, 2006 - v1
Checksum:i406F144C22F5787F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA. Translation: ABE57843.1.
RefSeqiWP_011505789.1. NC_007963.1.
YP_572542.1. NC_007963.1.

Genome annotation databases

EnsemblBacteriaiABE57843; ABE57843; Csal_0481.
KEGGicsa:Csal_0481.
PATRICi21444694. VBIChrSal113723_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA. Translation: ABE57843.1.
RefSeqiWP_011505789.1. NC_007963.1.
YP_572542.1. NC_007963.1.

3D structure databases

ProteinModelPortaliQ1R0B5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290398.Csal_0481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE57843; ABE57843; Csal_0481.
KEGGicsa:Csal_0481.
PATRICi21444694. VBIChrSal113723_0496.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

Entry informationi

Entry nameiSYI_CHRSD
AccessioniPrimary (citable) accession number: Q1R0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: April 29, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.