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Q1R0B5

- SYI_CHRSD

UniProt

Q1R0B5 - SYI_CHRSD

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Csal_0481
Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylate By similarity
Binding sitei612 – 6121ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Csal_0481
OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifieri290398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
ProteomesiUP000000239: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022057Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290398.Csal_0481.

Structurei

3D structure databases

ProteinModelPortaliQ1R0B5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi609 – 6135"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1R0B5-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYKHTLNL PETDFPMRGM LPKREPGRVS QWQDMNLYQR LRDARAGREV    50
FVLHDGPPYA NGSIHIGHAV NKILKDIVVK SKSLAGYDAP YVPGWDCHGL 100
PIEHKVETTH GKHLEADEAR GLCREYAGAQ IEGQKTDFVR LGVVGDWENP 150
YRTMDFANEA GEIRALAKMV EGGYVFKGLK PVNWCFDCGS ALAEAEVEYA 200
DKKSDAIDVA FAAAEPEALA SAFGLASLDK PAAIVIWTTT PWTIPANQAL 250
NVHPDFTYAL VDTGELLLVL AEELVESCLT RYGLEGDVVA TAAGASLEFI 300
TFRHPFYERV APVYLADYVT VEEGSTGIVH SAPSYGLDDF MTCREHGMSF 350
DDMLNPVQGN GVYRDDLPLF GGQMIWKANP HIVDTLREVN ALMAHTPITH 400
SYMHCWRHKT PVIYRATAQW FVGMDREDDQ GRTLRQRALD GVEATQFIPA 450
WGKARLHSMI ANRPDWCISR QRNWGVPIPF FMHKQTGEWH PRTVELMEDV 500
ARRVEAEGID AWFRLDAREL LGDEADQYDK VTDTLDVWFD SGTTHRHVMR 550
GSHPMGHDQG PRADLYLEGS DQHRGWFHSS LLTGCAIDGH APYKALLTHG 600
FTVDEKGRKM SKSMGNVIAP QQVMDKLGAD ILRLWVASTD YSGEMAVSDE 650
ILKRTADVYR RIRNTSRFLL ANLNGFAPER DAVAFEDMLA LDQWVVDRAA 700
QLQARIQGAY AEYRFRDVYQ QVHDFCAHEL GGFYLDVIKD RQYTTQADSR 750
SRRSCQTALY HVVEALVRWV APILSFTAEE IYEVIPGTRG DSVLLEEYYP 800
HLATLEANAA MGREFWSRVL TVKQAVNKCL EDARNAKVVR NSLAAEVTLY 850
ADDSLRETLT QLGEELRFVL LTSEVHLASL EDAGSAQAEA SELEGLKVAV 900
VSSPHAKCER CWHHRADVGT HAADPNLCGR CLSNLPEGPG ETRRYA 946
Length:946
Mass (Da):106,075
Last modified:May 16, 2006 - v1
Checksum:i406F144C22F5787F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000285 Genomic DNA. Translation: ABE57843.1.
RefSeqiYP_572542.1. NC_007963.1.

Genome annotation databases

EnsemblBacteriaiABE57843; ABE57843; Csal_0481.
GeneIDi4026505.
KEGGicsa:Csal_0481.
PATRICi21444694. VBIChrSal113723_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000285 Genomic DNA. Translation: ABE57843.1 .
RefSeqi YP_572542.1. NC_007963.1.

3D structure databases

ProteinModelPortali Q1R0B5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290398.Csal_0481.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE57843 ; ABE57843 ; Csal_0481 .
GeneIDi 4026505.
KEGGi csa:Csal_0481.
PATRICi 21444694. VBIChrSal113723_0496.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

Entry informationi

Entry nameiSYI_CHRSD
AccessioniPrimary (citable) accession number: Q1R0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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