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Q1QYD5 (BIOB_CHRSD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Csal_1167
OrganismChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]
Taxonomic identifier290398 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Biotin synthase HAMAP-Rule MF_01694
PRO_0000381305

Sites

Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding781Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1151Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1461Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2061Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2781Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1QYD5 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: F07B8D1EAEAD4A5A

FASTA37041,001
        10         20         30         40         50         60 
MTAQSRDPAW TDASPTFQPT MRHDWSLEEI EALFALPFND LLFRAQQVHR AHFDPNAVQV 

        70         80         90        100        110        120 
STLLSIKTGA CPEDCKYCPQ SGHYNTGLGK EKLLEIEKVV EQARAAKAAG ASRFCMGAAW 

       130        140        150        160        170        180 
RSPREKDLRV VTEMVGRVKA LGLETCMTLG MVDVDQARRL AEAGLDYYNH NLDTSPDYYG 

       190        200        210        220        230        240 
EIITTRTYAD RLETLANVRE AGMKVCSGGI LGMGEAPRDR AALLQQLVRL DPHPESVPIN 

       250        260        270        280        290        300 
MLVKVPGTPM ENVEDMDPLT FIRAIAVARI LMPKSHVRLS AGREQMDEST QALAFLAGAN 

       310        320        330        340        350        360 
SIFYGDTLLT TGNPQVERDR ALFDKLGLHP EPSDPHADDA HRDDEQAEIA LAHAIQRQRD 

       370 
DALFYDATRG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000285 Genomic DNA. Translation: ABE58523.1.
RefSeqYP_573222.1. NC_007963.1.

3D structure databases

ProteinModelPortalQ1QYD5.
SMRQ1QYD5. Positions 22-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290398.Csal_1167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE58523; ABE58523; Csal_1167.
GeneID4028106.
KEGGcsa:Csal_1167.
PATRIC21446114. VBIChrSal113723_1182.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CHRSD
AccessionPrimary (citable) accession number: Q1QYD5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways