Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14MagnesiumUniRule annotation1
Metal bindingi73MagnesiumUniRule annotation1
Metal bindingi145MagnesiumUniRule annotation1
Metal bindingi148MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCSAL290398:G1GJB-1896-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Csal_1846
OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifieri290398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
Proteomesi
  • UP000000239 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003324141 – 186Crossover junction endodeoxyribonuclease RuvCAdd BLAST186

Proteomic databases

PRIDEiQ1QWG0

Interactioni

Protein-protein interaction databases

STRINGi290398.Csal_1846

Structurei

3D structure databases

ProteinModelPortaliQ1QWG0
SMRiQ1QWG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Q1QWG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGEGSATIL LGIDPGSRIT GYGVVDVATP TPRYVASGCI RIKSDQLAQK
60 70 80 90 100
LAQVYAGIGE LIGLHRPHEI AIEQVFMSKN ADSALKLGQA RGTAIVCAAN
110 120 130 140 150
HGLEVFEYAP TQIKQAVTGT GAATKEQVQH MVTAILGLDG VPQADAADAL
160 170 180
AIALTHAYAR QGRSLPPSRG RRRSGSRQRW RDYRPS
Length:186
Mass (Da):19,745
Last modified:May 16, 2006 - v1
Checksum:i5A28D98D5F368054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA Translation: ABE59198.1
RefSeqiWP_011507144.1, NC_007963.1

Genome annotation databases

EnsemblBacteriaiABE59198; ABE59198; Csal_1846
KEGGicsa:Csal_1846

Similar proteinsi

Entry informationi

Entry nameiRUVC_CHRSD
AccessioniPrimary (citable) accession number: Q1QWG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health