Reviewed, UniProtKB/Swiss-Prot Q1QVG5 (MURD_CHRSD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Csal_2192

Organism

Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]

Taxonomic identifier

290398 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257179
Regions
	Nucleotide binding	118 – 124	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1QVG5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 16D3EA8BA4530CCB	FASTA	455	48,490
	10 20 30 40 50 60 MPRVPAAHTL VIGLGVSGQA IARHLSRRGE PFMVADTRES PAGLEAFRAA HPGVDVVCGP 70 80 90 100 110 120 LEALDMQEAR EIVLSPGVDP RTPGLIDYVD HPGSGPEVVG EMALFVRECR SPIAAITGSN 130 140 150 160 170 180 AKSTVTTLLG EMARESGWKT AVGGNLGTPA LDLLDESPDA ELFVLELSSF QLETTPWLGA 190 200 210 220 230 240 DTAAFLNLSE DHLDRHGDMQ GYRAAKQRIF RGARHAVVNA EDPATWPDAP SCAVTRFTTD 250 260 270 280 290 300 MPESGEWGIV DHDGERWLAQ GRAAIMPVGQ VRMPGRHNHA NALAALAMGA HLGLSREAMC 310 320 330 340 350 360 RVLERFPGLP HRGEFIVERE GVRWINDSKG TNVGATLAAI AGIGSDLEGR LILLAGGDGK 370 380 390 400 410 420 GADFSPLAEP LAHHAREAIV FGRDAERLEQ ALSARLPVTR VADLAAAMQR ARTIARAGDT 430 440 450 VLLSPACASL DQFPNYMARG EAFRQWLATD GEAAC

References

[1]

"Complete sequence of Chromohalobacter salexigens DSM 3043." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Saunders E., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Csonka L.N., O'Conner K., Vreeland R.H., Oren A., Vargas C., Nieto J., Arahal D.R., Goodner B., Wheeler C., Hall P., Ewing A., Benson L., McBeath D., Canovas D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000285 Genomic DNA. Translation: ABE59543.1.
RefSeq	YP_574242.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4026686.
GenomeReviews	Gene locus Csal_2192 in contig CP000285_GR.
KEGG	csa:Csal_2192.
NMPDR	fig|290398.4.peg.2862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1QVG5.
OMA	Q1QVG5. SEDHLDR.
Enzyme and pathway databases
BioCyc	CSAL290398:CSAL_2192-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHRSD

Accession

Primary (citable) accession number: Q1QVG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)