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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei76Charge relay systemUniRule annotation1
Active sitei151Charge relay systemUniRule annotation1
Active sitei175Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSAL290398:G1GJB-2305-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Csal_2242
OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifieri290398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
Proteomesi
  • UP000000239 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000158211 – 486Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST486

Proteomic databases

PRIDEiQ1QVB5

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi290398.Csal_2242

Structurei

3D structure databases

ProteinModelPortaliQ1QVB5
SMRiQ1QVB5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiPITQNVE
OrthoDBiPOG091H00O9

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q1QVB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHDKTLTELA AGLRAGEFSS RELCQSALDR IQRLDGDLNS FISVTAEQAL
60 70 80 90 100
ADADAADQAR ASGDAAALAG LPLALKDIFC TQGVRTSCGS KMLDSFVAPY
110 120 130 140 150
DATVVEKIRQ AGGVSLGKTN MDEFAMGSSN ENSFYGPVKN PWDLSAVPGG
160 170 180 190 200
SSGGSAAAVA AGLVPAALGT DTGGSIRQPA AFCGITGLKP TYGRVSRYGM
210 220 230 240 250
VAYASSLDQG GPMARTAADC ALLLETIAGH DVRDSTSVAR VVPDYSAELD
260 270 280 290 300
TSLAGLKIGL PKEYFGDGLD PEVETAVREA IKVYESLGAK VCEVSLPHTH
310 320 330 340 350
LAIPAYYVIA PAEASSNLSR YDGVRFGHRC EDPTDLEDLY KRTRAEGFGE
360 370 380 390 400
EVKRRILIGT HTLSEGFFDA YYLKAQQVRR LIRQDFLDAF DEVDVLMGPT
410 420 430 440 450
APTPAFDLGA QKDPVSMYLQ DIYTIAVNLA GIPGISVPAG FAGNRPVGLQ
460 470 480
VLAPHFAEAQ LLGVAHQFQQ ATDWHLKRPA FGKEMA
Length:486
Mass (Da):51,549
Last modified:May 16, 2006 - v1
Checksum:iA13C8443413DF486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA Translation: ABE59593.1
RefSeqiWP_011507539.1, NC_007963.1

Genome annotation databases

EnsemblBacteriaiABE59593; ABE59593; Csal_2242
KEGGicsa:Csal_2242

Similar proteinsi

Entry informationi

Entry nameiGATA_CHRSD
AccessioniPrimary (citable) accession number: Q1QVB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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