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Reviewed, UniProtKB/Swiss-Prot Q1QUR0 (LEU3_CHRSD)

Last modified June 16, 2009. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH
Gene names
Name: leuB
Ordered Locus Names: Csal_2451
OrganismChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]
Taxonomic identifier290398 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter

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Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3593593-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000250112

Regions

Nucleotide binding281 – 29313NAD By similarity

Sites

Metal binding2231Magnesium or manganese By similarity
Metal binding2471Magnesium or manganese By similarity
Metal binding2511Magnesium or manganese By similarity
Binding site961Substrate By similarity
Binding site1061Substrate By similarity
Binding site1341Substrate By similarity
Binding site2231Substrate By similarity
Site1411Important for catalysis By similarity
Site1911Important for catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q1QUR0-1 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: A6569DD652F0CF1B

FASTA35938,363
        10         20         30         40         50         60 
MSRKILVLPG DGIGPEITRE AVKILNACRE AGLEASIEEG LVGGAGIDAH GVPLPDETLA 

        70         80         90        100        110        120 
SAKAADAVLL GAVGGPQWDK IEDLSKRPEK GLLGLRKNLN LFGNLRPALL YPQLASASSL 

       130        140        150        160        170        180 
KPEIVAGLDI MIVRELTGGI YFGQPRGIEE RDGERVGYNT YIYAEHEIER IGRVAFEMAR 

       190        200        210        220        230        240 
ERGGKLCSVD KANVLEATIL WREVMERLAP EYPDVALSHM YVDNAAMQLV RAPKQFDVIV 

       250        260        270        280        290        300 
TGNMFGDILS DAAAMLTGSI GMLPSASLNE QRQGMYEPCH GSAPDIAGQG IANPLATILS 

       310        320        330        340        350 
VAMMLRYSLE APQLAERIER AVGTVLDQGL RTADIAFEGT APVSTQAMGD AVLAAFQAQ

« Hide

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References

[1]"Complete sequence of Chromohalobacter salexigens DSM 3043."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Saunders E., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Csonka L.N., O'Conner K., Vreeland R.H., Oren A., Vargas C., Nieto J., Arahal D.R., Goodner B., Wheeler C., Hall P., Ewing A., Benson L., McBeath D., Canovas D., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000285 Genomic DNA. Translation: ABE59798.1. Different initiation.
RefSeqYP_574497.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4026971.
GenomeReviewsGene locus Csal_2451 in contig CP000285_GR.
KEGGcsa:Csal_2451.
NMPDRfig|290398.4.peg.3092.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ1QUR0.

Enzyme and pathway databases

BioCycCSAL290398:CSAL_2451-MON.

Family and domain databases

HAMAPMF_01033.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. leuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLEU3_CHRSD
AccessionPrimary (citable) accession number: Q1QUR0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents