Reviewed, UniProtKB/Swiss-Prot Q1QTP6 (DAPA_CHRSD)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Csal_2816

Organism

Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) [Complete proteome] [HAMAP]

Taxonomic identifier

290398 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050177
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QTP6-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 89BB7BC3D57186C7	FASTA	294	31,496
	10 20 30 40 50 60 MITGSIVALA TPMKASGEID WEALRRLVGF HLDNGTDAIV AAGTTGEPTT MSFAEHFDVI 70 80 90 100 110 120 RCVVEEVAGR IPVIAGTGAN ATSEAVELAR YAREVGADLC LSVAPYYNKP TQEGLYQHFK 130 140 150 160 170 180 AVAEGGGLPV ILYNVPGRTC SDIYNETVFR LAELENIVGL KDATGNLERA EELVDRLKGS 190 200 210 220 230 240 DFALYSGDDP TACDFMLMGG HGDISVCANV APRAMHELCE AAVAGDDERA HQINTRLMPL 250 260 270 280 290 HTALGVEANP IPVKWALHRM GLAEQGIRLP MTWLSDKYHS TVSEALQLAG VTEG

References

[1]

"Complete sequence of Chromohalobacter salexigens DSM 3043." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Saunders E., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Csonka L.N., O'Conner K., Vreeland R.H., Oren A., Vargas C., Nieto J., Arahal D.R., Goodner B., Wheeler C., Hall P., Ewing A., Benson L., McBeath D., Canovas D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000285 Genomic DNA. Translation: ABE60162.1.
RefSeq	YP_574861.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QTP6.
Genome annotation databases
GeneID	4028501.
GenomeReviews	Gene locus Csal_2816 in contig CP000285_GR.
KEGG	csa:Csal_2816.
NMPDR	fig|290398.4.peg.2169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1QTP6.
OMA	VAPRLMH.
Enzyme and pathway databases
BioCyc	CSAL290398:CSAL_2816-MON.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CHRSD

Accession

Primary (citable) accession number: Q1QTP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)