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Protein

Malate synthase G

Gene

glcB

Organism
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi442 – 4421MagnesiumUniRule annotation
Binding sitei442 – 4421GlyoxylateUniRule annotation
Metal bindingi470 – 4701MagnesiumUniRule annotation
Binding sitei551 – 5511Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei650 – 6501Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Csal_3154
OrganismiChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifieri290398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter
ProteomesiUP000000239: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 742742Malate synthase GPRO_1000056901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei636 – 6361Cysteine sulfenic acid (-SOH)UniRule annotation
Modified residuei707 – 7071Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290398.Csal_3154.

Structurei

3D structure databases

ProteinModelPortaliQ1QSR0.
SMRiQ1QSR0. Positions 1-739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni467 – 4704Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiIQIDAND.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1QSR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRTTRHRL QVASQLDRFI NDEALPGTGV DPEAFWAGFD ALVHELVPTN
60 70 80 90 100
RDLLAERERL QDELDAWHKA NPGPVRDMAA YRAFLKDIGY LVEAPAKVQA
110 120 130 140 150
TTANVDDEIA VQAGPQLVVP VSNARYALNA ANARWGSLYD ALYGTDVIPE
160 170 180 190 200
TDGAEKSQGY NPKRGEKVIA YARGVLDQAA PLAEGTHAEA SAYALRDGKL
210 220 230 240 250
VVTLQGGGET GLADPAQLVG YRGEAQAPTA VLLANHGLHL EVQFDATHPI
260 270 280 290 300
GKTDPAHVKD VLVEAAVSTI MDCEDSVAAV DADDKTLVYR NWLGLMKGDL
310 320 330 340 350
EERFDKGGKT VTRALNPDRD YTVPGGGELR LPGRSLLFVR NVGHLMTTPA
360 370 380 390 400
VLDGDGNEIP EGMLDGVVTS LLAIHDLKKG DGAAPSATAP EAKRNSRTGS
410 420 430 440 450
VYIVKPKMHG PREVAFANSL FMRIEDMLGL PRDTLKMGIM DEERRTSINL
460 470 480 490 500
DACIHEAASR VAFINTGFLD RTGDEMHTAM EAGPMLRKGE MKGTKWIAAY
510 520 530 540 550
EKNNVQTGLA CGLRGRAQIG KGMWAMPELM AAMLEQKIGH PQAGATTAWV
560 570 580 590 600
PSPTAAVLHA LHYHQVDVAT IQRELEAKPG GDFLDDLLTV PVVESAASGA
610 620 630 640 650
NKSPSWSDDE IQQELDNNCQ GILGYVVRWV EHGVGCSKVP DIHDVGLMED
660 670 680 690 700
RATLRISSQH IANWLHHGIV SEARVRETLE RMAKVVDDQN AHDPDYTPMT
710 720 730 740
SHLAESCAFQ AASDLIFKGR EQPAGYTEPL LHHWRAVHKA KS
Length:742
Mass (Da):80,564
Last modified:May 16, 2006 - v1
Checksum:i52D50E6BAB982917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA. Translation: ABE60498.1.
RefSeqiWP_011508444.1. NC_007963.1.
YP_575197.1. NC_007963.1.

Genome annotation databases

EnsemblBacteriaiABE60498; ABE60498; Csal_3154.
GeneIDi4028621.
KEGGicsa:Csal_3154.
PATRICi21450192. VBIChrSal113723_3174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000285 Genomic DNA. Translation: ABE60498.1.
RefSeqiWP_011508444.1. NC_007963.1.
YP_575197.1. NC_007963.1.

3D structure databases

ProteinModelPortaliQ1QSR0.
SMRiQ1QSR0. Positions 1-739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290398.Csal_3154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE60498; ABE60498; Csal_3154.
GeneIDi4028621.
KEGGicsa:Csal_3154.
PATRICi21450192. VBIChrSal113723_3174.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiIQIDAND.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 3043 / ATCC BAA-138 / NCIMB 13768.

Entry informationi

Entry nameiMASZ_CHRSD
AccessioniPrimary (citable) accession number: Q1QSR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: March 4, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.