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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80S-adenosyl-L-methionineUniRule annotation1
Binding sitei105S-adenosyl-L-methionineUniRule annotation1
Binding sitei132S-adenosyl-L-methionineUniRule annotation1
Active sitei154By similarity1
Binding sitei154S-adenosyl-L-methionineUniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Binding sitei190SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Nham_0027
OrganismiNitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Taxonomic identifieri323097 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000001953 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881891 – 255tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST255

Interactioni

Protein-protein interaction databases

STRINGi323097.Nham_0027.

Structurei

3D structure databases

ProteinModelPortaliQ1QS68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1QS68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAASDPHN PRSSADDTAS PRCESGQGSF FGRRKGHKLR PHQNGLIEHL
60 70 80 90 100
LPRLSLDPGA SGPSDPATLF GRPVKEMRIE IGFGGGEHLV AEALAFPRTG
110 120 130 140 150
FIGCEPYVNG MAKILSQIEA HDIGNIRLLA GDASELLAWL PRASLARIDL
160 170 180 190 200
IHPDPWPKRR HWKRRFVQDA TVAEMARVLR PGGEFRFVSD IADYCAWTLS
210 220 230 240 250
HLARSPGFLW LAERATDWHN PWPDYTMTRY GRKAEREGRR AAYLRFRRES

DAAQA
Length:255
Mass (Da):28,542
Last modified:May 16, 2006 - v1
Checksum:i161ABC34515233F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE60929.1.
RefSeqiWP_011508636.1. NC_007964.1.

Genome annotation databases

EnsemblBacteriaiABE60929; ABE60929; Nham_0027.
KEGGinha:Nham_0027.
PATRICi22687353. VBINitHam61822_0720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE60929.1.
RefSeqiWP_011508636.1. NC_007964.1.

3D structure databases

ProteinModelPortaliQ1QS68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323097.Nham_0027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE60929; ABE60929; Nham_0027.
KEGGinha:Nham_0027.
PATRICi22687353. VBINitHam61822_0720.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_NITHX
AccessioniPrimary (citable) accession number: Q1QS68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.