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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Nitrobacter hamburgensis (strain X14 / DSM 10229)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi374 – 3818GTPUniRule annotation
Nucleotide bindingi421 – 4255GTPUniRule annotation
Nucleotide bindingi475 – 4784GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNHAM323097:GHP7-31-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:Nham_0031
OrganismiNitrobacter hamburgensis (strain X14 / DSM 10229)
Taxonomic identifieri323097 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
ProteomesiUP000001953: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868Translation initiation factor IF-2PRO_1000008288Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi323097.Nham_0031.

Structurei

3D structure databases

ProteinModelPortaliQ1QS64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini365 – 535171tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni374 – 3818G1By similarity
Regioni399 – 4035G2By similarity
Regioni421 – 4244G3By similarity
Regioni475 – 4784G4By similarity
Regioni511 – 5133G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076907.
KOiK02519.
OMAiAHQKREK.
OrthoDBiEOG67HJSV.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1QS64-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDTKNPGDK TLSVGPSKTL TLKPRVEQGT VRQSFSHGRT KQVVVEKRGK
60 70 80 90 100
RRIGGDAPAA EPVAASEPTV ARAPRIPVRP APPAASRANS GVVLRTLTED
110 120 130 140 150
ERTARASALA EAKIRAEEER RIAEAEAARR NSKEGIEQAE REAAEARRKA
160 170 180 190 200
EEERHRQEEE AKRKAELEAK RRFGESETKA APAKTTTTTT RAAPPARPAA
210 220 230 240 250
VAADGSDEEE APRLVRRPGG GPARPVVAPK PAAKPAPAKQ RGRLTLVTAL
260 270 280 290 300
SADDVRERSI ASFRRRTQRL KGHASNEPKE KLIREVIVPE AITIQELANR
310 320 330 340 350
MAERAVDVIR MLMKQGAMHK ITDVIDADTA QLIAEELGHT VKRVAASDVE
360 370 380 390 400
EGLFDADDDS TVVEPRSPVV TVMGHVDHGK TSLLDAVRHA NVVSGEAGGI
410 420 430 440 450
TQHIGAYQVT SPDGKKITFI DTPGHAAFTA MRARGAKVTD IVVLVVAADD
460 470 480 490 500
GVMPQTVEAI NHAKAAKVPM IIAINKIDKP DAKPERVRTE LLQHEVQVES
510 520 530 540 550
MGGQVIDVEV SAKNKTNLDK LLEMISLQAD LLDLKTNASR PAEGTVIEAK
560 570 580 590 600
LDRGRGPVAT VLVQRGTLRV GDIIVAGAEM GRVRALISDQ GETVDEAGPS
610 620 630 640 650
VPVEVLGFNG PPEAGDRLAV VENEARARQV TSYRAHQKRE NAAASSSGMR
660 670 680 690 700
GSLEQMMSQL KTAGRKDFPL VIKADVQGSL EAILGSLEKL GTEEVAARIL
710 720 730 740 750
HAGVGGISES DVTLAEGFNA AIIGFSVRAN KEAAAAAKRN GIEIRYYNII
760 770 780 790 800
YDLVDDVKKA MSGLLAPTLR ETMLGNAEIL EVFNISKVGK VAGCRVTDGN
810 820 830 840 850
VERGANVRLI RENVVVHEGK LSTLKRFKDE VKEVQSGQEC GMAFESYHDM
860
RVGDVIECYR VETIQRSL
Length:868
Mass (Da):93,501
Last modified:May 16, 2006 - v1
Checksum:i8CCA8FA9CA43480D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE60933.1.
RefSeqiYP_575393.1. NC_007964.1.

Genome annotation databases

EnsemblBacteriaiABE60933; ABE60933; Nham_0031.
GeneIDi4029798.
KEGGinha:Nham_0031.
PATRICi22687361. VBINitHam61822_0724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE60933.1.
RefSeqiYP_575393.1. NC_007964.1.

3D structure databases

ProteinModelPortaliQ1QS64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323097.Nham_0031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE60933; ABE60933; Nham_0031.
GeneIDi4029798.
KEGGinha:Nham_0031.
PATRICi22687361. VBINitHam61822_0724.

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076907.
KOiK02519.
OMAiAHQKREK.
OrthoDBiEOG67HJSV.

Enzyme and pathway databases

BioCyciNHAM323097:GHP7-31-MONOMER.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: X14 / DSM 10229.

Entry informationi

Entry nameiIF2_NITHX
AccessioniPrimary (citable) accession number: Q1QS64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: February 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.