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Q1QS64 (IF2_NITHX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Nham_0031
OrganismNitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]
Taxonomic identifier323097 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000008288

Regions

Nucleotide binding374 – 3818GTP By similarity
Nucleotide binding421 – 4255GTP By similarity
Nucleotide binding475 – 4784GTP By similarity
Region368 – 517150G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
Q1QS64 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 8CCA8FA9CA43480D

FASTA86893,501
        10         20         30         40         50         60 
MVDTKNPGDK TLSVGPSKTL TLKPRVEQGT VRQSFSHGRT KQVVVEKRGK RRIGGDAPAA 

        70         80         90        100        110        120 
EPVAASEPTV ARAPRIPVRP APPAASRANS GVVLRTLTED ERTARASALA EAKIRAEEER 

       130        140        150        160        170        180 
RIAEAEAARR NSKEGIEQAE REAAEARRKA EEERHRQEEE AKRKAELEAK RRFGESETKA 

       190        200        210        220        230        240 
APAKTTTTTT RAAPPARPAA VAADGSDEEE APRLVRRPGG GPARPVVAPK PAAKPAPAKQ 

       250        260        270        280        290        300 
RGRLTLVTAL SADDVRERSI ASFRRRTQRL KGHASNEPKE KLIREVIVPE AITIQELANR 

       310        320        330        340        350        360 
MAERAVDVIR MLMKQGAMHK ITDVIDADTA QLIAEELGHT VKRVAASDVE EGLFDADDDS 

       370        380        390        400        410        420 
TVVEPRSPVV TVMGHVDHGK TSLLDAVRHA NVVSGEAGGI TQHIGAYQVT SPDGKKITFI 

       430        440        450        460        470        480 
DTPGHAAFTA MRARGAKVTD IVVLVVAADD GVMPQTVEAI NHAKAAKVPM IIAINKIDKP 

       490        500        510        520        530        540 
DAKPERVRTE LLQHEVQVES MGGQVIDVEV SAKNKTNLDK LLEMISLQAD LLDLKTNASR 

       550        560        570        580        590        600 
PAEGTVIEAK LDRGRGPVAT VLVQRGTLRV GDIIVAGAEM GRVRALISDQ GETVDEAGPS 

       610        620        630        640        650        660 
VPVEVLGFNG PPEAGDRLAV VENEARARQV TSYRAHQKRE NAAASSSGMR GSLEQMMSQL 

       670        680        690        700        710        720 
KTAGRKDFPL VIKADVQGSL EAILGSLEKL GTEEVAARIL HAGVGGISES DVTLAEGFNA 

       730        740        750        760        770        780 
AIIGFSVRAN KEAAAAAKRN GIEIRYYNII YDLVDDVKKA MSGLLAPTLR ETMLGNAEIL 

       790        800        810        820        830        840 
EVFNISKVGK VAGCRVTDGN VERGANVRLI RENVVVHEGK LSTLKRFKDE VKEVQSGQEC 

       850        860 
GMAFESYHDM RVGDVIECYR VETIQRSL 

« Hide

References

[1]"Complete sequence of chromosome of Nitrobacter hamburgensis X14."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: X14 / DSM 10229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000319 Genomic DNA. Translation: ABE60933.1.
RefSeqYP_575393.1. NC_007964.1.

3D structure databases

ProteinModelPortalQ1QS64.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323097.Nham_0031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE60933; ABE60933; Nham_0031.
GeneID4029798.
KEGGnha:Nham_0031.
PATRIC22687361. VBINitHam61822_0724.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMAGAMHKIT.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycNHAM323097:GHP7-31-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_NITHX
AccessionPrimary (citable) accession number: Q1QS64
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries