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Q1QRZ8 (SYL_NITHX) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Nham_0098
OrganismNitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]
Taxonomic identifier323097 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 878878Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009379

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif630 – 6345"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1QRZ8 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: E201CAD309F8241A

FASTA87898,481
        10         20         30         40         50         60 
MTSDRYNARD AEPRWQAAWD QQAIFATKND DPREKYYVLE MFPYPSGRIH IGHVRNYTLG 

        70         80         90        100        110        120 
DVIARYMRAK GYNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIKAMK KQLRSIGLSL 

       130        140        150        160        170        180 
DWAREFATCD PSYYKHQQKM FLDFLRAGLA EREKRKINWD PVDMTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GAVVEQREMN QWVFKITKYS QELLEALDTL DRWPDKVRLM QRNWIGRSEG LLLRFALDPA 

       250        260        270        280        290        300 
TTPNGEGELK IFTTRPDTLF GAKFMAIAPD HPLAQAAAKD NPALAEFIAE CKRRGTAQAE 

       310        320        330        340        350        360 
IDTAEKMGFD TGIRAIHPFD PDWTLPVYVA NFILMEYGTG AIFGCPAHDQ RDLDFVNKYG 

       370        380        390        400        410        420 
LGNTPVVCPE GQDPKTLVIT DTAYDGDGRM INSRFLDGMT AEAAKKEVAK RLESEMRGNM 

       430        440        450        460        470        480 
PVGERKVNFR LRDWGISRQR YWGCPIPVIH CPKCDVVPVP ENDLPVTLPE DVTFDKPGNA 

       490        500        510        520        530        540 
LDHHPTWKHV TCPQCGARAT RETDTMDTFV DSSWYFARFT DPWNETAPTT PEIANRMMPV 

       550        560        570        580        590        600 
DQYIGGVEHA ILHLLYSRFF TRAMKATGHL SMDEPFKGMF TQGMVVHETY RKADGGWASP 

       610        620        630        640        650        660 
DEVGIEAVGN GRRATLISTG EPVEIGAVEK MSKSKRNTVD PDDIIGSYGA DTARWFMLSD 

       670        680        690        700        710        720 
SPPDRDVIWS EEGVQGASRF MQRLWRLVNE SAEAGKAAPR DKPATFGTDA LALRKAAHGA 

       730        740        750        760        770        780 
LDKVSTGIER LHFNVCLANI REFANTLAET LARFGTRTSD LAPDIAWSLR EAATILVQLF 

       790        800        810        820        830        840 
SPMMPHLSEE CWHALGHTGL VSEARWPQIE RDLLVEDTVT LPVQVNGKKR GEVTVASSAP 

       850        860        870 
NPEIETAVLA LDAVRQALGG KPARKIIIVP QRIVNVVG 

« Hide

References

[1]"Complete sequence of chromosome of Nitrobacter hamburgensis X14."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: X14 / DSM 10229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000319 Genomic DNA. Translation: ABE60999.1.
RefSeqYP_575459.1. NC_007964.1.

3D structure databases

ProteinModelPortalQ1QRZ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323097.Nham_0098.

Proteomic databases

PRIDEQ1QRZ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE60999; ABE60999; Nham_0098.
GeneID4029599.
KEGGnha:Nham_0098.
PATRIC22687513. VBINitHam61822_0799.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNHAM323097:GHP7-99-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NITHX
AccessionPrimary (citable) accession number: Q1QRZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries