true2009-07-282024-03-27103BIOB_NITHXComplete sequence of chromosome of Nitrobacter hamburgensis X14.Copeland A.Lucas S.Lapidus A.Barry K.Detter J.C.Glavina del Rio T.Hammon N.Israni S.Dalin E.Tice H.Pitluck S.Chain P.Malfatti S.Shin M.Vergez L.Schmutz J.Larimer F.Land M.Hauser L.Kyrpides N.Ivanova N.Ward B.Arp D.Klotz M.Stein L.O'Mullan G.Starkenburg S.Sayavedra L.Poret-Peterson A.T.Gentry M.E.Bruce D.Richardson P.US DOE Joint Genome Institute2006-03EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]DSM 10229 / NCIMB 13809 / X14BacteriaRadical_SAMAldolase class IBioBAldolase_TIMBATS_domBiotin_synth/BioABBiotin_synthaseElp3/MiaA/NifB-like_rSAMrSAMbioBBIOTIN SYNTHASEBIOTIN SYNTHASE, MITOCHONDRIALBATSRadical_SAMBiotin_synthbiotin_synthasebiotin_synthase_likeBATSElp3Radical SAM enzymesRADICAL_SAMBiotin synthase2.8.1.6bioBNham_0277Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.Homodimer.Belongs to the radical SAM superfamily. Biotin synthase family.Biotin synthase373271340Radical SAM core53272Disordered216872751121432032764Fe-4S-S-AdoMet2006-05-16137327b639a7c6b2d7fea6c8d80eceea42f100MDAASVSFGSGHDLSSQPRHDWTRSEAETLYNLPFADLIFQAQTLHRRHFDPNHVETANLLSIKTGGCPEDCGYCSQSAHYDSGVKATKLMDREAVIETARRARDAGASRFCMAAAWRNPKDRDLDRVCDMVSAVKELGLETCATLGMLTPDQARRLHDAGLDFYNHNVDTSPEFYGNIITTRTMQDRIDTLAHAREAGLKVCCGGIIGLGEQVGDRLGMLMLLANLPAHPESVPINMWNEVKSVPVNDTAERPDPIALVRMIAVARIMMPRSVVRLSAGRQYMTDELQALCFLAGANSIFIGDVLLTTKNPQTARDAALLDRLGIRSRLDDAKAAAPPHtruetrue