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Q1QRH1

- BIOB_NITHX

UniProt

Q1QRH1 - BIOB_NITHX

Protein

Biotin synthase

Gene

bioB

Organism
Nitrobacter hamburgensis (strain X14 / DSM 10229)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi112 – 1121Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi276 – 2761Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciNHAM323097:GHP7-281-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Nham_0277
    OrganismiNitrobacter hamburgensis (strain X14 / DSM 10229)
    Taxonomic identifieri323097 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
    ProteomesiUP000001953: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Biotin synthasePRO_0000381502Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323097.Nham_0277.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1QRH1.
    SMRiQ1QRH1. Positions 19-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiFEIANEC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1QRH1-1 [UniParc]FASTAAdd to Basket

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    MDAASVSFGS GHDLSSQPRH DWTRSEAETL YNLPFADLIF QAQTLHRRHF    50
    DPNHVETANL LSIKTGGCPE DCGYCSQSAH YDSGVKATKL MDREAVIETA 100
    RRARDAGASR FCMAAAWRNP KDRDLDRVCD MVSAVKELGL ETCATLGMLT 150
    PDQARRLHDA GLDFYNHNVD TSPEFYGNII TTRTMQDRID TLAHAREAGL 200
    KVCCGGIIGL GEQVGDRLGM LMLLANLPAH PESVPINMWN EVKSVPVNDT 250
    AERPDPIALV RMIAVARIMM PRSVVRLSAG RQYMTDELQA LCFLAGANSI 300
    FIGDVLLTTK NPQTARDAAL LDRLGIRSRL DDAKAAAPPH 340
    Length:340
    Mass (Da):37,327
    Last modified:May 16, 2006 - v1
    Checksum:iA966C435E63CF96E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000319 Genomic DNA. Translation: ABE61176.1.
    RefSeqiWP_011508880.1. NC_007964.1.
    YP_575636.1. NC_007964.1.

    Genome annotation databases

    EnsemblBacteriaiABE61176; ABE61176; Nham_0277.
    GeneIDi4031157.
    KEGGinha:Nham_0277.
    PATRICi22687909. VBINitHam61822_0994.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000319 Genomic DNA. Translation: ABE61176.1 .
    RefSeqi WP_011508880.1. NC_007964.1.
    YP_575636.1. NC_007964.1.

    3D structure databases

    ProteinModelPortali Q1QRH1.
    SMRi Q1QRH1. Positions 19-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323097.Nham_0277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE61176 ; ABE61176 ; Nham_0277 .
    GeneIDi 4031157.
    KEGGi nha:Nham_0277.
    PATRICi 22687909. VBINitHam61822_0994.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi FEIANEC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci NHAM323097:GHP7-281-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: X14 / DSM 10229.

    Entry informationi

    Entry nameiBIOB_NITHX
    AccessioniPrimary (citable) accession number: Q1QRH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3