Q1QR91 (GLPK_NITHX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Nham_0360

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Glycerol kinase HAMAP MF_00186		PRO_1000020752
Regions
	Nucleotide binding	412 – 416	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QR91 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: E41C9C995FC5FCFD	FASTA	503	53,996
	10 20 30 40 50 60 MVTHVLAIDQ GTTSSRAILF RADTSIAAIA QQEFPQHFPA SGWVEHEPED IWTSTVATCR 70 80 90 100 110 120 EAMNKAGVTP KDIAAIGITN QRETTVVWDR ATGQAMHRAV VWQDRRTADI CARLKAEGHE 130 140 150 160 170 180 PAISAKTGLI VDPYFSGTKI AWILDSVPGA RARASRGDLL FGTVDCYLLW RLTGGKVHAT 190 200 210 220 230 240 DATNASRTLL FDIHTGQWDE ELLKILRVPR AMLPEVKDSS AHFGDSVADL FGAPISIRGI 250 260 270 280 290 300 AGDQQAAVVG QACFAPGMIK STYGTGCFAL LNTGATPVAS KNKLLTTIAY QLNGKRTYAL 310 320 330 340 350 360 EGSIFVAGSA VQWLRDGLGL IKQASETGPL ADRSDATQSV YLVPAFVGMG APYWNPNVRG 370 380 390 400 410 420 ALFGLTRNTG PAELAHAALE SVCYQTFDLW AAMRADWPDA AAAHTVLRVD GGMTASDWTM 430 440 450 460 470 480 QRLADLLDAP VDRPVIQETT ALGAAYLAGL SAGVYPEPSK FADNWRLERR FKPAMSAATR 490 500 TRKLKGWAAA VRGVLASDGG EGR

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: X14 / DSM 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000319 Genomic DNA. Translation: ABE61256.1.
RefSeq	YP_575716.1. NC_007964.1.
3D structure databases
ProteinModelPortal	Q1QR91.
SMR	Q1QR91. Positions 5-492.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QR91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4030246.
GenomeReviews	Gene locus Nham_0360 in contig CP000319_GR.
KEGG	nha:Nham_0360.
NMPDR	fig|323097.3.peg.543.
PATRIC	22688099. VBINitHam61822_1087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	CYQTRDL.
PhylomeDB	Q1QR91.
ProtClustDB	CLSK818559.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_0360-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_NITHX

Accession

Primary (citable) accession number: Q1QR91

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program