Reviewed, UniProtKB/Swiss-Prot Q1QPR8 (HGD_NITHX)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	Nham_0921

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Homogentisate 1,2-dioxygenase		PRO_1000019531
Sites
	Metal binding	346	1	Iron By similarity
	Metal binding	352	1	Iron By similarity
	Metal binding	382	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QPR8-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 1E4007C93C156DCA	FASTA	448	49,674
	10 20 30 40 50 60 MNTSFDPVAA NSNSANVTPG YMSGFGNSFE TEALPGALPI GRNSPQRCAY GLYAEQLSGS 70 80 90 100 110 120 PFTAPRGANE RSWLYRIRPS VRHSGHFVKI DAKLWRTAPC LEHDMPLAQL RWDPTPIPED 130 140 150 160 170 180 ELTFVQSVRT MTTAGDAHTQ TGMAAHIYLI TRSMVDQHFY NADGEMLFVP QGGSLRFVTE 190 200 210 220 230 240 FGVIDTEPGE IAVIPRGVKF RVEIPSGPAR GYLCENYGGA FTLPERGPIG ANCLANSRDF 250 260 270 280 290 300 LTPVAAYEDD DKPTELFVKW GGALWSTALP HSPIDVVAWH GNYAPYKYDL RTFSPIGAIG 310 320 330 340 350 360 FDHPDPSIFT VLTSPSEIAG TANIDFVIFP ERWVVAENTF RPPWYHMNVM SEFMGLIYGV 370 380 390 400 410 420 YDAKPQGFVP GGISLHNCML PHGPDREAFD HASNTELKPV KLTGTLAFMF ETRFPQRVTE 430 440 YAATSDALQD DYADCWQGLE RRFDPTRP

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000319 Genomic DNA. Translation: ABE61779.1.
RefSeq	YP_576239.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4031915.
GenomeReviews	Gene locus Nham_0921 in contig CP000319_GR.
KEGG	nha:Nham_0921.
NMPDR	fig|323097.3.peg.895.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1QPR8.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_0921-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_NITHX

Accession

Primary (citable) accession number: Q1QPR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	November 4, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)