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Q1QNB1 (GLGB_NITHX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Nham_1464
OrganismNitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]
Taxonomic identifier323097 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7167161,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260669

Sites

Active site3981Nucleophile By similarity
Active site4511Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1QNB1 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: D8523F4B6EDE1D6C

FASTA71680,882
        10         20         30         40         50         60 
MIELSSEARA IVEGRHSDPF HYLGRHREND RQVVRAYLPE ASDVRVVDEH GGETTLTRIH 

        70         80         90        100        110        120 
DAGLFLGDLP NGAKRYRLRA RFGADTVELE DPYRFPPILS DFDLYLLGEG TDQRLYDKLG 

       130        140        150        160        170        180 
AHPITMDGVG GTGFVVLAPN ARAVAVVGDF NCWDRRRHPM RVRGNGYWEL FIPGAQTGDR 

       190        200        210        220        230        240 
YKFAITDKSG SLLPLKSDPM AFAAEVRPNT ASIVVDEAAL PCPTPAAATI NARRAPISIY 

       250        260        270        280        290        300 
EVHLGSWRRG DDNRWLSYRE LADTLPRYAS SLGFTHIELM PVNEHPFDGS WGYQPTGLYA 

       310        320        330        340        350        360 
PTSRFGSPDD FAHLVDACHR EGLGVLLDWV PGHFPDDPHG LGHFDGTAFY EHANPLQGRH 

       370        380        390        400        410        420 
LDWDTLIYNY GRTEVVNFLV ANALFWLDRY GLDGLRVDAV ASMLYLDYSR AEGGWIPNKH 

       430        440        450        460        470        480 
GGRENLEAIA FLRRFNREVF AKFPHATTIA EESTAWPQVS RPIEFGGLGF GYKWNMGWMH 

       490        500        510        520        530        540 
DTLDYIGKDP IYRKHHHGQI LFGLHYAFSE NFILPLSHDE VVHEKRSILG RMPGDDWQRF 

       550        560        570        580        590        600 
ANLRAYYAFM FGHPGKKLMF MGAEFGQERE WNHDHALDWH LLDQARHQGV QAVVRDLNAL 

       610        620        630        640        650        660 
YRDVPALHEL DCDPAGFEWI MANDADRSVF AWMRKGESGR ARCLVIANFT PTVHRSYRVR 

       670        680        690        700        710 
VPFAGKWREA LNTDSAHYGG SNVGNNGVVE TLDGVIPELS LTLPPLAVIF LVPERA 

« Hide

References

[1]"Complete sequence of chromosome of Nitrobacter hamburgensis X14."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: X14 / DSM 10229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000319 Genomic DNA. Translation: ABE62286.1.
RefSeqYP_576746.1. NC_007964.1.

3D structure databases

ProteinModelPortalQ1QNB1.
SMRQ1QNB1. Positions 111-715.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323097.Nham_1464.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE62286; ABE62286; Nham_1464.
GeneID4032865.
KEGGnha:Nham_1464.
PATRIC22690559. VBINitHam61822_2305.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHERCPHA.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycNHAM323097:GHP7-1485-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_NITHX
AccessionPrimary (citable) accession number: Q1QNB1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 16, 2006
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries