Q1QN44 (RPOB_NITHX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	Nham_1531

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	1380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1380	1380	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300358

Sequences

	Sequence		Length	Mass (Da)
	Q1QN44 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 7D1B2045EC393C5A	FASTA	1,380	154,219
	10 20 30 40 50 60 MVQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVDEPEGGRL DEGLQAVFKS 70 80 90 100 110 120 VFPINDFSGT SQLEFVRYEF EAPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETAAKS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFVVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEEILST FYKKIIYKRA 250 260 270 280 290 300 KSGSNADGWR VPYDPVRFRG YSTLNDLIDA DSGKVVLEAG KKLTVRAARQ LQEKGLKALR 310 320 330 340 350 360 MSDEELVGMY LAEDLVNPKT GEIYAEAGEE ITEKSLKALN EHGYKELPLL DIDHVNVGPY 370 380 390 400 410 420 IRNTLSADKN LTREDALFDI YRVMRPGEPP TLESAQNMFQ SLFFDSERYD LSAVGRVKMN 430 440 450 460 470 480 MRLDLDAPDT YRTLRKEDIL AVIKTLVDLR DGKGEIDDID HLGNRRVRSV GELMENQYRV 490 500 510 520 530 540 GLLRMERAIK ERMSSVDIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH 550 560 570 580 590 600 KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF 610 620 630 640 650 660 VETPYRKVKD GRVTDEVVYL SAMEEGRYHV AQANLPLDNR GRFTEDLVVC RHAGEVLPVT 670 680 690 700 710 720 SDKVDFMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMEG 730 740 750 760 770 780 VVARDSGAAI AARRSGIIDQ IDATRVVIRA TEDLDPTKSG VDIYRLMKYQ RSNQSTCINQ 790 800 810 820 830 840 RPLVKVGDVV KKGDIIADGP STDLGELALG RNVLVAFMPW NGYNFEDSIL LSERIVKEDV 850 860 870 880 890 900 FTSIHIEEFE VMARDTKLGP EEITRDIPNV SEEALKNLDE AGIVYIGAEV RAGDILVGKI 910 920 930 940 950 960 TPKGESPMTP EEKLLRAIFG EKASDVRDTS LRVPPGVQGT IVEVRVFNRH GVDKDERALA 970 980 990 1000 1010 1020 IEREEIERLA KDRDDEQAIL DRNVYGRLAD LLENRQGIAG PKGFKKDTKI TRSVIEEYPK 1030 1040 1050 1060 1070 1080 SQWWLFASPN DKLMAEIEAM RKQYDESKKG LEQRFLDKVE KLQRGDELPP GVMKMVKVFV 1090 1100 1110 1120 1130 1140 AVKRKIQPGD KMAGRHGNKG VVSKIVPIED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE 1150 1160 1170 1180 1190 1200 THLGWACAGL GRRIGQAVDA YLASAKQETK PLKETLKKVY GDNETIKSLN DHELVELGRN 1210 1220 1230 1240 1250 1260 LSRGVPIATP VFDGAKEADI EQMLELAGMD KSGQSTVYDG RTGDTFDRKV TVGYIYMLKL 1270 1280 1290 1300 1310 1320 HHLVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS 1330 1340 1350 1360 1370 1380 DDVAGRTKVY EAIVRGDDTF EAGIPESFNV LVKEMRSLGL NVDLHNSKIG SATPTSEAAE

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: X14 / DSM 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000319 Genomic DNA. Translation: ABE62353.1.
RefSeq	YP_576813.1. NC_007964.1.
3D structure databases
HSSP	HSSP built from PDB template 2GHO based on UniProtKB Q9KWU7.
ProteinModelPortal	Q1QN44.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QN44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4032624.
GenomeReviews	Gene locus Nham_1531 in contig CP000319_GR.
KEGG	nha:Nham_1531.
NMPDR	fig|323097.3.peg.1449.
PATRIC	22690717. VBINitHam61822_2383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q1QN44.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_1531-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_NITHX

Accession

Primary (citable) accession number: Q1QN44

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program