Reviewed, UniProtKB/Swiss-Prot Q1QMM1 (SYQ_NITHX)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Nham_1710

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095494
Regions
	Motif	36 – 46	11	"HIGH" region HAMAP MF_00126
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QMM1-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 7D76EAEF0A412495	FASTA	558	63,038
	10 20 30 40 50 60 MTDESTADAG RDFIRDIVAA DLASGKHKSV VTRFPPEPNG YLHLGHAKSI CLNFGIAEEF 70 80 90 100 110 120 GGCCHLRFDD TNPTKEEQEY IDAIQRDVRW LGFDWGKNLH YASDYFEQLY AWAQHLVRAG 130 140 150 160 170 180 KAYVDDQSQE DIRLARGTLT EPGRDSPFRD RPVDENLDLF ARMRAGEFPN GARVLRARID 190 200 210 220 230 240 MASGNINLRD PVLYRILHAP HPRTGTAWNI YPSYDFAHGQ SDSIEHITHS ICTLEFEDHR 250 260 270 280 290 300 PLYDWFLDNL PVPSRPHQYE FARLNVTHTL LSKRILTELV RGGHVAGWDD PRMPTLAGLQ 310 320 330 340 350 360 RRGVPAEALR EFIKRIGVAK ANSTVDVGML DFAIRETLNK SAPRRMAVLR PLKIVIENYP 370 380 390 400 410 420 EGESEELEAV NHPDDPSRGT RRIRFGRELY IEQDDFMENP PKKFFRLSPG REVRLRYAYF 430 440 450 460 470 480 VTCREVVKNA AGDVVELRCT YDPATKGGNA PDGRKVKATM HWVSAAEAVP AEIRLYGHLF 490 500 510 520 530 540 ATAQPDAANF ASELNPQSLE TLSGMVEPAL ARDDTDGAVQ FERQGYFCRD RDSSPGRLVF 550 NRTVGLRDTW AKVAAAGS

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000319 Genomic DNA. Translation: ABE62526.1.
RefSeq	YP_576986.1.
3D structure databases
SMR	Q1QMM1. Positions 12-549.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QMM1.
Genome annotation databases
GeneID	4030310.
GenomeReviews	Gene locus Nham_1710 in contig CP000319_GR.
KEGG	nha:Nham_1710.
NMPDR	fig|323097.3.peg.2233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
PhylomeDB	Q1QMM1.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_1710-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_NITHX

Accession

Primary (citable) accession number: Q1QMM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 16, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)