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Q1QMC9 (TRMFO_NITHX) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:Nham_1804
OrganismNitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]
Taxonomic identifier323097 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346367

Regions

Nucleotide binding15 – 206FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1QMC9 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 0BE127CFBCA17572

FASTA47751,271
        10         20         30         40         50         60 
MTQIELSCSA VHIIGAGLAG SEAAWQVANH GVRVVLHEMR PHRMTAAHQT GGLAELVCSN 

        70         80         90        100        110        120 
SFRSDDAANN AVGLLHAEMR RLGSLIMRCA DANQVPAGGA LAVDRDGFSS AVTKALDDHP 

       130        140        150        160        170        180 
LIEINRTEVD GLPPADWRNV IVATGPLTSA PLAAAIRALT DESALAFFDA IAPIVHRDSI 

       190        200        210        220        230        240 
DMSKAWFQSR YDKVGPGGTG ADYINCPMTE AQYHAFVDAL IEGEKVDFKD WEIDTPYFDG 

       250        260        270        280        290        300 
CLPIEIMAER GRETLRHGPM KPVGLTNPHD PTVKPYAIVQ LRQDNRLGTL YNIVGFQTKL 

       310        320        330        340        350        360 
KHGAQTRVFR TIPGLETAEF ARLGGLHRNT FLNSPKLLDT QLRLRAEPRL RFAGQMTGCE 

       370        380        390        400        410        420 
GYVESAAIGL IAGLYAASDA RARSLAAPPP TTALGALLGH ITGGHIESID AGPRSFQPMN 

       430        440        450        460        470 
INFGLFPPLA NPPAKKPDGT RLRGNEKTIA KKQAISARAL ADLDRWIADA LRVAAAA 

« Hide

References

[1]"Complete sequence of chromosome of Nitrobacter hamburgensis X14."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: X14 / DSM 10229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000319 Genomic DNA. Translation: ABE62618.1.
RefSeqYP_577078.1. NC_007964.1.

3D structure databases

ProteinModelPortalQ1QMC9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323097.Nham_1804.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE62618; ABE62618; Nham_1804.
GeneID4032981.
KEGGnha:Nham_1804.
PATRIC22691296. VBINitHam61822_2668.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMAWEMRAAG.
OrthoDBEOG6J74VT.

Enzyme and pathway databases

BioCycNHAM323097:GHP7-1833-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_NITHX
AccessionPrimary (citable) accession number: Q1QMC9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families