Q1QIN5 (LPXK_NITHX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Nham_3177

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000291217
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1QIN5 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: C4A27D8A4C66AD00	FASTA	335	35,518
	10 20 30 40 50 60 MHEPAFWHRP SSLLSRLLMP VGALYGAVAA RRLMRTGMRA GVPVICVGNY HVGGAGKTPT 70 80 90 100 110 120 VIALAGILRS LGETPVVLSR GYGGRLHGPV HVDPHRHTAA DVGDEPLMMA WTIPVIVSRQ 130 140 150 160 170 180 RAAGIAPARA LGASVILMDD GFQNPALAKD ISLIVIDRAR GLGNGQVFPA GPLRAPLPPQ 190 200 210 220 230 240 LARTDALVIV GFGPAADDVA ASIGARGGLV LPARLIPDDA SVVALRGRRV YAFAGIGDPQ 250 260 270 280 290 300 RFFRSLRACG IDVAAERAFP DHHPFSQRDV ADLQTAAERD GLTLVTTEKD LARLRNSEDL 310 320 330 AAFAQAVVAF AVTLAFDDEA VLRSFLTDRI GRARR

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: X14 / DSM 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000319 Genomic DNA. Translation: ABE63912.1.
RefSeq	YP_578372.1. NC_007964.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QIN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4029543.
GenomeReviews	Gene locus Nham_3177 in contig CP000319_GR.
KEGG	nha:Nham_3177.
NMPDR	fig|323097.3.peg.2349.
PATRIC	22694333. VBINitHam61822_4166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
PhylomeDB	Q1QIN5.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_3177-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_NITHX

Accession

Primary (citable) accession number: Q1QIN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program