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Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei273Acetyl-CoAUniRule annotation1
Binding sitei310Acetyl-CoAUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei628Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Nham_3565
OrganismiNitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Taxonomic identifieri323097 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569131 – 727Malate synthase GAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiQ1QHK3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323097.Nham_3565

Structurei

3D structure databases

ProteinModelPortaliQ1QHK3
SMRiQ1QHK3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q1QHK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIDAHGLK IAPVLFDFIA REATPRTGIA PDAFWAGLAA IVRDLGPRNR
60 70 80 90 100
ELLAVRDTLQ AQIDGWHRAN KSRPFDMAAY TAFLKEIGYL LPEPATHAVE
110 120 130 140 150
TANIDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIAHE
160 170 180 190 200
ANEARGYDKA RGDKVIAKAK AFLDSAAPLS AGSHADVAGY SVVNGHLSAK
210 220 230 240 250
LRSGDATGLK TIAQFAGYQG AADAPSAILL VNHGLHIDIR IDRADRIGKD
260 270 280 290 300
DPAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNTL GLMNGTLTDT
310 320 330 340 350
FEKGGKTVTR ALNADRIYTS ANGKEIALHG RSLLLMRNVG HHMVTDAVLD
360 370 380 390 400
ENGAEIPEGM LDAAVSSLLA IHDLMGISKT RNSRTGSVYI VKPKMHGPDE
410 420 430 440 450
VALTCELFAR VETMLGLPEN TLKIGIMDEE RRTTVNLKTC IQNASKRVCF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKNEMKA QPWMRAYEDW NVDIGLIDGL
510 520 530 540 550
PGHAQIGKGM WAAPDRMADM LAQKVDHPQA GATTAWVPSP TAATLHALHY
560 570 580 590 600
HQVDVLKQQQ ELKAGGQRAK LSDILTVPVS QSNWAPDDVR LEIDNNCQGI
610 620 630 640 650
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHQNVVTS
660 670 680 690 700
DQVMDSLKRM AVVVDKQNAG DPLYTPMAPT FDGVAFKAAC DLVFKGREQP
710 720
NGYTEYILTE RRRQAKAESK GESEAAG
Length:727
Mass (Da):78,879
Last modified:May 16, 2006 - v1
Checksum:iBAD4A93C140936FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA Translation: ABE64294.1
RefSeqiWP_011511935.1, NC_007964.1

Genome annotation databases

EnsemblBacteriaiABE64294; ABE64294; Nham_3565
KEGGinha:Nham_3565

Similar proteinsi

Entry informationi

Entry nameiMASZ_NITHX
AccessioniPrimary (citable) accession number: Q1QHK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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