Q1QHK3 (MASZ_NITHX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Nham_3565

Organism

Nitrobacter hamburgensis (strain X14 / DSM 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

323097 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Malate synthase G HAMAP MF_00641		PRO_1000056913
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	628	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QHK3 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: BAD4A93C140936FD	FASTA	727	78,879
	10 20 30 40 50 60 MTRIDAHGLK IAPVLFDFIA REATPRTGIA PDAFWAGLAA IVRDLGPRNR ELLAVRDTLQ 70 80 90 100 110 120 AQIDGWHRAN KSRPFDMAAY TAFLKEIGYL LPEPATHAVE TANIDDEIGK ICGPQLVVPL 130 140 150 160 170 180 TNARYALNAA NARWGSLYDA LYGTDAIAHE ANEARGYDKA RGDKVIAKAK AFLDSAAPLS 190 200 210 220 230 240 AGSHADVAGY SVVNGHLSAK LRSGDATGLK TIAQFAGYQG AADAPSAILL VNHGLHIDIR 250 260 270 280 290 300 IDRADRIGKD DPAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNTL GLMNGTLTDT 310 320 330 340 350 360 FEKGGKTVTR ALNADRIYTS ANGKEIALHG RSLLLMRNVG HHMVTDAVLD ENGAEIPEGM 370 380 390 400 410 420 LDAAVSSLLA IHDLMGISKT RNSRTGSVYI VKPKMHGPDE VALTCELFAR VETMLGLPEN 430 440 450 460 470 480 TLKIGIMDEE RRTTVNLKTC IQNASKRVCF INTGFLDRTG DEIHTSMEAG PMIRKNEMKA 490 500 510 520 530 540 QPWMRAYEDW NVDIGLIDGL PGHAQIGKGM WAAPDRMADM LAQKVDHPQA GATTAWVPSP 550 560 570 580 590 600 TAATLHALHY HQVDVLKQQQ ELKAGGQRAK LSDILTVPVS QSNWAPDDVR LEIDNNCQGI 610 620 630 640 650 660 LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHQNVVTS DQVMDSLKRM 670 680 690 700 710 720 AVVVDKQNAG DPLYTPMAPT FDGVAFKAAC DLVFKGREQP NGYTEYILTE RRRQAKAESK GESEAAG

References

[1]

"Complete sequence of chromosome of Nitrobacter hamburgensis X14." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: X14 / DSM 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000319 Genomic DNA. Translation: ABE64294.1.
RefSeq	YP_578754.1. NC_007964.1.
3D structure databases
ProteinModelPortal	Q1QHK3.
SMR	Q1QHK3. Positions 3-719.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QHK3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4032602.
GenomeReviews	Gene locus Nham_3565 in contig CP000319_GR.
KEGG	nha:Nham_3565.
NMPDR	fig|323097.3.peg.3554.
PATRIC	22695181. VBINitHam61822_4585.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	Q1QHK3.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	NHAM323097:NHAM_3565-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_NITHX

Accession

Primary (citable) accession number: Q1QHK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program