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Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei273Acetyl-CoAUniRule annotation1
Binding sitei310Acetyl-CoAUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei628Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Nham_3565
OrganismiNitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Taxonomic identifieri323097 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000001953 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569131 – 727Malate synthase GAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323097.Nham_3565.

Structurei

3D structure databases

ProteinModelPortaliQ1QHK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1QHK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIDAHGLK IAPVLFDFIA REATPRTGIA PDAFWAGLAA IVRDLGPRNR
60 70 80 90 100
ELLAVRDTLQ AQIDGWHRAN KSRPFDMAAY TAFLKEIGYL LPEPATHAVE
110 120 130 140 150
TANIDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIAHE
160 170 180 190 200
ANEARGYDKA RGDKVIAKAK AFLDSAAPLS AGSHADVAGY SVVNGHLSAK
210 220 230 240 250
LRSGDATGLK TIAQFAGYQG AADAPSAILL VNHGLHIDIR IDRADRIGKD
260 270 280 290 300
DPAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNTL GLMNGTLTDT
310 320 330 340 350
FEKGGKTVTR ALNADRIYTS ANGKEIALHG RSLLLMRNVG HHMVTDAVLD
360 370 380 390 400
ENGAEIPEGM LDAAVSSLLA IHDLMGISKT RNSRTGSVYI VKPKMHGPDE
410 420 430 440 450
VALTCELFAR VETMLGLPEN TLKIGIMDEE RRTTVNLKTC IQNASKRVCF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKNEMKA QPWMRAYEDW NVDIGLIDGL
510 520 530 540 550
PGHAQIGKGM WAAPDRMADM LAQKVDHPQA GATTAWVPSP TAATLHALHY
560 570 580 590 600
HQVDVLKQQQ ELKAGGQRAK LSDILTVPVS QSNWAPDDVR LEIDNNCQGI
610 620 630 640 650
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHQNVVTS
660 670 680 690 700
DQVMDSLKRM AVVVDKQNAG DPLYTPMAPT FDGVAFKAAC DLVFKGREQP
710 720
NGYTEYILTE RRRQAKAESK GESEAAG
Length:727
Mass (Da):78,879
Last modified:May 16, 2006 - v1
Checksum:iBAD4A93C140936FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE64294.1.
RefSeqiWP_011511935.1. NC_007964.1.

Genome annotation databases

EnsemblBacteriaiABE64294; ABE64294; Nham_3565.
KEGGinha:Nham_3565.
PATRICi22695181. VBINitHam61822_4585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE64294.1.
RefSeqiWP_011511935.1. NC_007964.1.

3D structure databases

ProteinModelPortaliQ1QHK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323097.Nham_3565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE64294; ABE64294; Nham_3565.
KEGGinha:Nham_3565.
PATRICi22695181. VBINitHam61822_4585.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_NITHX
AccessioniPrimary (citable) accession number: Q1QHK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.