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Q1QHK3

- MASZ_NITHX

UniProt

Q1QHK3 - MASZ_NITHX

Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter hamburgensis (strain X14 / DSM 10229)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei273 – 2731Acetyl-CoAUniRule annotation
    Binding sitei310 – 3101Acetyl-CoAUniRule annotation
    Active sitei337 – 3371Proton acceptorUniRule annotation
    Binding sitei337 – 3371GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei628 – 6281Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciNHAM323097:GHP7-3621-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Nham_3565
    OrganismiNitrobacter hamburgensis (strain X14 / DSM 10229)
    Taxonomic identifieri323097 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
    ProteomesiUP000001953: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_1000056913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323097.Nham_3565.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1QHK3.
    SMRiQ1QHK3. Positions 3-719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1QHK3-1 [UniParc]FASTAAdd to Basket

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    MTRIDAHGLK IAPVLFDFIA REATPRTGIA PDAFWAGLAA IVRDLGPRNR    50
    ELLAVRDTLQ AQIDGWHRAN KSRPFDMAAY TAFLKEIGYL LPEPATHAVE 100
    TANIDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIAHE 150
    ANEARGYDKA RGDKVIAKAK AFLDSAAPLS AGSHADVAGY SVVNGHLSAK 200
    LRSGDATGLK TIAQFAGYQG AADAPSAILL VNHGLHIDIR IDRADRIGKD 250
    DPAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNTL GLMNGTLTDT 300
    FEKGGKTVTR ALNADRIYTS ANGKEIALHG RSLLLMRNVG HHMVTDAVLD 350
    ENGAEIPEGM LDAAVSSLLA IHDLMGISKT RNSRTGSVYI VKPKMHGPDE 400
    VALTCELFAR VETMLGLPEN TLKIGIMDEE RRTTVNLKTC IQNASKRVCF 450
    INTGFLDRTG DEIHTSMEAG PMIRKNEMKA QPWMRAYEDW NVDIGLIDGL 500
    PGHAQIGKGM WAAPDRMADM LAQKVDHPQA GATTAWVPSP TAATLHALHY 550
    HQVDVLKQQQ ELKAGGQRAK LSDILTVPVS QSNWAPDDVR LEIDNNCQGI 600
    LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHQNVVTS 650
    DQVMDSLKRM AVVVDKQNAG DPLYTPMAPT FDGVAFKAAC DLVFKGREQP 700
    NGYTEYILTE RRRQAKAESK GESEAAG 727
    Length:727
    Mass (Da):78,879
    Last modified:May 16, 2006 - v1
    Checksum:iBAD4A93C140936FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000319 Genomic DNA. Translation: ABE64294.1.
    RefSeqiYP_578754.1. NC_007964.1.

    Genome annotation databases

    EnsemblBacteriaiABE64294; ABE64294; Nham_3565.
    GeneIDi4032602.
    KEGGinha:Nham_3565.
    PATRICi22695181. VBINitHam61822_4585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000319 Genomic DNA. Translation: ABE64294.1 .
    RefSeqi YP_578754.1. NC_007964.1.

    3D structure databases

    ProteinModelPortali Q1QHK3.
    SMRi Q1QHK3. Positions 3-719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323097.Nham_3565.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE64294 ; ABE64294 ; Nham_3565 .
    GeneIDi 4032602.
    KEGGi nha:Nham_3565.
    PATRICi 22695181. VBINitHam61822_4585.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci NHAM323097:GHP7-3621-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: X14 / DSM 10229.

    Entry informationi

    Entry nameiMASZ_NITHX
    AccessioniPrimary (citable) accession number: Q1QHK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3