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Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter hamburgensis (strain X14 / DSM 10229)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei273 – 2731Acetyl-CoAUniRule annotation
Binding sitei310 – 3101Acetyl-CoAUniRule annotation
Active sitei337 – 3371Proton acceptorUniRule annotation
Binding sitei337 – 3371GlyoxylateUniRule annotation
Metal bindingi429 – 4291MagnesiumUniRule annotation
Binding sitei429 – 4291GlyoxylateUniRule annotation
Metal bindingi457 – 4571MagnesiumUniRule annotation
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei628 – 6281Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciNHAM323097:GHP7-3621-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Nham_3565
OrganismiNitrobacter hamburgensis (strain X14 / DSM 10229)
Taxonomic identifieri323097 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
ProteomesiUP000001953 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GPRO_1000056913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323097.Nham_3565.

Structurei

3D structure databases

ProteinModelPortaliQ1QHK3.
SMRiQ1QHK3. Positions 3-719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni454 – 4574Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1QHK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRIDAHGLK IAPVLFDFIA REATPRTGIA PDAFWAGLAA IVRDLGPRNR
60 70 80 90 100
ELLAVRDTLQ AQIDGWHRAN KSRPFDMAAY TAFLKEIGYL LPEPATHAVE
110 120 130 140 150
TANIDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIAHE
160 170 180 190 200
ANEARGYDKA RGDKVIAKAK AFLDSAAPLS AGSHADVAGY SVVNGHLSAK
210 220 230 240 250
LRSGDATGLK TIAQFAGYQG AADAPSAILL VNHGLHIDIR IDRADRIGKD
260 270 280 290 300
DPAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNTL GLMNGTLTDT
310 320 330 340 350
FEKGGKTVTR ALNADRIYTS ANGKEIALHG RSLLLMRNVG HHMVTDAVLD
360 370 380 390 400
ENGAEIPEGM LDAAVSSLLA IHDLMGISKT RNSRTGSVYI VKPKMHGPDE
410 420 430 440 450
VALTCELFAR VETMLGLPEN TLKIGIMDEE RRTTVNLKTC IQNASKRVCF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKNEMKA QPWMRAYEDW NVDIGLIDGL
510 520 530 540 550
PGHAQIGKGM WAAPDRMADM LAQKVDHPQA GATTAWVPSP TAATLHALHY
560 570 580 590 600
HQVDVLKQQQ ELKAGGQRAK LSDILTVPVS QSNWAPDDVR LEIDNNCQGI
610 620 630 640 650
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHQNVVTS
660 670 680 690 700
DQVMDSLKRM AVVVDKQNAG DPLYTPMAPT FDGVAFKAAC DLVFKGREQP
710 720
NGYTEYILTE RRRQAKAESK GESEAAG
Length:727
Mass (Da):78,879
Last modified:May 16, 2006 - v1
Checksum:iBAD4A93C140936FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE64294.1.
RefSeqiYP_578754.1. NC_007964.1.

Genome annotation databases

EnsemblBacteriaiABE64294; ABE64294; Nham_3565.
KEGGinha:Nham_3565.
PATRICi22695181. VBINitHam61822_4585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000319 Genomic DNA. Translation: ABE64294.1.
RefSeqiYP_578754.1. NC_007964.1.

3D structure databases

ProteinModelPortaliQ1QHK3.
SMRiQ1QHK3. Positions 3-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323097.Nham_3565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE64294; ABE64294; Nham_3565.
KEGGinha:Nham_3565.
PATRICi22695181. VBINitHam61822_4585.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciNHAM323097:GHP7-3621-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: X14 / DSM 10229.

Entry informationi

Entry nameiMASZ_NITHX
AccessioniPrimary (citable) accession number: Q1QHK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.