Q1QEW1 (RLMF_PSYCK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Pcryo_0008

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.
Sequence caution	The sequence ABE73792.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349941

Sequences

	Sequence		Length	Mass (Da)
	Q1QEW1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: C6CA35B6AFEAEFE0	FASTA	345	38,728
	10 20 30 40 50 60 MTSKPMNRRP TANNHRNRSP ETAKKLGQLH PRNPHQGRYD FEVLTRALPE LAKHTITNPK 70 80 90 100 110 120 GESTINFSDS AAVRVLNQAL LANYYGVKFW DIPEGYLCPP IPGRADYIHY IADLLAQTTH 130 140 150 160 170 180 VNKDNTPPTG KEIHALDIGT GASAIYPIVG SQSYGWRFTA SDIDPISVNT AALICETNPK 190 200 210 220 230 240 LKSAVKVKLQ TEPKFIFKNI IGRQDYFDVV VCNPPFHASL EEAMEANSRK QHNLQRHRGK 250 260 270 280 290 300 NENEQISRSS TKPGNAAQNL NFGGQHKELW SEGGEIAFLT KMAKESQDFA EHVGWFTTLV 310 320 330 340 SKSENVKPLQ LLLKQLGVAQ MRIIEMSQGQ KSTRILAWRF NTDEE

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000323 Genomic DNA. Translation: ABE73792.1. Different initiation.
RefSeq	YP_579276.1. NC_007969.1.
3D structure databases
ProteinModelPortal	Q1QEW1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QEW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4034876.
GenomeReviews	Gene locus Pcryo_0008 in contig CP000323_GR.
KEGG	pcr:Pcryo_0008.
NMPDR	fig|335284.3.peg.432.
PATRIC	23059399. VBIPsyCry128170_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	PCRY335284:PCRYO_0008-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSYCK

Accession

Primary (citable) accession number: Q1QEW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program