Q1QE80 (POTA_PSYCK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	Pcryo_0239

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter › Psychrobacter cryohalolentis

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286277
Regions
	Domain	22 – 252	231	ABC transporter
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1QE80 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: F73CC43E82A05544	FASTA	402	45,162
	10 20 30 40 50 60 MTESLLTPLT TPASANTDKV LLQLTGLKKT YDNTEVLKDI NLDIKHGEFI TLLGPSGCGK 70 80 90 100 110 120 TTLLRLIAGF EQPSAGAIYL DGVQMAGLPA DKRPVNTVFQ QYALFPHMTV AQNVAYGLKL 130 140 150 160 170 180 RKVPKVEIQT RVREMLAMVQ LEHLANRRPQ DLSGGQQQRV AIARAVINRP KLLLLDEPLS 190 200 210 220 230 240 ALDHKLRLQM QSELKRLQRE LGITFVFVTH DQEEALSMSD RIAVMKDGRF QQIGTPIEIY 250 260 270 280 290 300 ETPANLFTAK FIGETNLFKA EVKGVYPEQP DRDGRVTNGR IEVEVCEAQV QDGPTPVRNL 310 320 330 340 350 360 RRPDFANDVQ VGDIVNLLLR PEDLRIYDPN DKEHGGLLGR VIESNYKGST LDSIIELTNG 370 380 390 400 HIIKASEFFD EDDPSFDYKL NEGVKVSWVD GWEWVLPEED IH

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000323 Genomic DNA. Translation: ABE74023.1.
RefSeq	YP_579507.1. NC_007969.1.
3D structure databases
ProteinModelPortal	Q1QE80.
ModBase	Search...
Protein-protein interaction databases
STRING	335284.Pcryo_0239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE74023; ABE74023; Pcryo_0239.
GeneID	4035431.
KEGG	pcr:Pcryo_0239.
PATRIC	23059893. VBIPsyCry128170_0294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	RESETTF.
ProtClustDB	PRK09452.
Enzyme and pathway databases
BioCyc	PCRY335284:GHE9-305-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR005893. Sp_pt_ABC_ATP-bd. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit.
TIGRFAMs	TIGR01187. potA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_PSYCK

Accession

Primary (citable) accession number: Q1QE80

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program