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Q1QDA4 (SYL_PSYCK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pcryo_0566
OrganismPsychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]
Taxonomic identifier335284 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABE74349.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 919919Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334798

Regions

Motif83 – 9311"HIGH" region HAMAP-Rule MF_00049
Motif670 – 6745"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1QDA4 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 892790991B9E9351

FASTA919103,257
        10         20         30         40         50         60 
MLWHYKISPH LSVITDSEPT MTNAVTAETA NTNHTNNASS DKTQYQPQLI EAQQQAKWAT 

        70         80         90        100        110        120 
DKRFEVSNEP SDKPSRYMLS MFPYPSGKLH MGHVRNYTIS DVLSRYYRLK GYEVMQPMGW 

       130        140        150        160        170        180 
DGFGLPAENA AIANQTPPAK WTFENIDSMR AQLKLLGLSI DWSREFATCS PEYYQWEQWL 

       190        200        210        220        230        240 
FLQLYKKGLV YKKLATVNWD PVDNTVLANE QVIDGKGWRS GAMVEKRDIP MYYFNITDYA 

       250        260        270        280        290        300 
DELLDDLDQL EGHWPSEVIT MQRNWIGRSS GMEVHFPYEL AGQSNSLDVF TTRPDTLMGV 

       310        320        330        340        350        360 
TYVAVAAEHP LAQYASENNK AIADFCALCK KGSVAEADLA KAEKIGMDTG LTVTHPLTGE 

       370        380        390        400        410        420 
EVPVWVANYV LMSYGSGAVM AVPAHDERDY EFATKYNLPI KQVIDIPAGY FDDVEEGNEN 

       430        440        450        460        470        480 
CAYTERNTLV NSGEFDGMDF EQAFEAMLAK LEPQELAKKK IQYRLRDWGV SRQRYWGCPI 

       490        500        510        520        530        540 
PMVNCEHCGT VPVEEQDLPV VLPTDVVPDG RGNPLKNIPE FVNTTCPKCG NPAERETDTF 

       550        560        570        580        590        600 
DTFVESSWYY ARFASPHDTT NMVNKSAANK WLPVDQYIGG VEHAVMHLLY ARFFHKLMRD 

       610        620        630        640        650        660 
ENLVSGDEPF ANLMTQGMVL AGTFYRVNAD GSTTYYFTKD IDIDFNERGQ PIKAILKSDG 

       670        680        690        700        710        720 
QPVTIGKIEK MSKSKNNGVD PQITIDKYGA DTVRLYTLFT APADQTLEWS DDALKGPYNF 

       730        740        750        760        770        780 
VKKVWRIASE HIQALTDANL SLESLNSDAL NTDSLSKEAK ALRRKTHETI GKIDSDLGKR 

       790        800        810        820        830        840 
LALNTPVSSL MELANELSNF KASSEQELQV QHEALTDLLI MLSVYAPHIG EYLLEQLGLD 

       850        860        870        880        890        900 
TVTLNYPMVD ESALVQDMIT MVIQVNGKMR GKMDVAPNSD PEQLKAQARA IEGVAKFLTG 

       910 
EIKKEIVVPN KLVNIVVAG 

« Hide

References

[1]"Complete sequence of chromosome of Psychrobacter cryohalolentis K5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000323 Genomic DNA. Translation: ABE74349.1. Different initiation.
RefSeqYP_579833.1. NC_007969.1.

3D structure databases

ProteinModelPortalQ1QDA4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335284.Pcryo_0566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE74349; ABE74349; Pcryo_0566.
GeneID4033584.
KEGGpcr:Pcryo_0566.
PATRIC23060609. VBIPsyCry128170_0635.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPCRY335284:GHE9-590-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSYCK
AccessionPrimary (citable) accession number: Q1QDA4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries