Q1QCP8 (RLMKL_PSYCK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

1. 23S rRNA m2G2445 methyltransferase
   EC=2.1.1.173
   Alternative name(s):
   rRNA (guanine-N(2)-)-methyltransferase RlmL
2. 23S rRNA m7G2069 methyltransferase
   EC=2.1.1.264
   Alternative name(s):
   rRNA (guanine-N(7)-)-methyltransferase RlmK

Gene names

Name:	rlmL
Ordered Locus Names:	Pcryo_0772

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter › Psychrobacter cryohalolentis

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858
Catalytic activity	S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858 S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01858.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmKL family. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	23S rRNA (guanine(2445)-N(2))-methyltransferase activity Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: HAMAP rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858		PRO_0000366800
Regions
	Domain	68 – 183	116	THUMP

Sequences

	Sequence		Length	Mass (Da)
	Q1QCP8 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 3E4220E880B3D51D	FASTA	776	87,393
	10 20 30 40 50 60 MTETTALASS TASSQPSSQA SSQVLSLDLI ITCADGLEAP LQTELTSFGI ASEIKSTGRL 70 80 90 100 110 120 AVTGTLRDLY KICLWSRVAS RVLMLIKRKN INAEYDVAEQ LYGLAKSVNW TEQFSLEQTF 130 140 150 160 170 180 AIRLSVDKRV AVSQQFAMLR IKDAIADTFN EVYESRPNVD SKNPDFSVFA TVNDKQAELY 190 200 210 220 230 240 LDLSGTSLHR RGYRVAMTEA PLKENLAAAL LYSAGWHKKN EAGDTPFYNA LIDPMCGSGT 250 260 270 280 290 300 FIIEALLMHC DYAVGIDKAA NQFGFYEWQH HDAALWQEMI DDAQTRFRAA LEIANEQPDT 310 320 330 340 350 360 LPLIFGFDAD NGAIIATEKN LIAAGLQDLL PLLDIETRAL DQLSTLLKPL VADGRLSNPL 370 380 390 400 410 420 IITNPPYGER LGDEEMIKPL YQSIGLILQD SFAGSGIDPM LGILASHVEQ VDILPIREPK 430 440 450 460 470 480 TLRCHNGAIT VYFRYGTLIA GQTGNLVSRF EKREIEVEDG QDFINRLQKN LTRLKKLAKK 490 500 510 520 530 540 DNVSNIRVYN ADLPDFKVAI DLYGDYAHVQ EYAPPKTIPP ETAKKRFNLA LMGIREVFGI 550 560 570 580 590 600 NREQIFIKTR ARQSGNDQYS KQGNTEKRGK FYIAREDGAY LYVNFTDYLD TGLFIDHRNM 610 620 630 640 650 660 RARIKDNSRN KSVLNLFAYT CTASVHAALA GAKKVTSVDL SQNYLDWGKQ NFALNGLNVS 670 680 690 700 710 720 SNKYQFVAAD IFEWIKDNTE QFDIIFIDPP TFSNSKKFQG TFDVQRDHAA LINRAMNRLT 730 740 750 760 770 ADGILYFSNN FTRFELDEQL TERYDIIDIT QKTIGFDFDI KKPIHQSFEI RHRQSL

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000323 Genomic DNA. Translation: ABE74555.1.
RefSeq	YP_580039.1. NC_007969.1.
3D structure databases
ProteinModelPortal	Q1QCP8.
ModBase	Search...
Protein-protein interaction databases
STRING	335284.Pcryo_0772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE74555; ABE74555; Pcryo_0772.
GeneID	4034006.
KEGG	pcr:Pcryo_0772.
PATRIC	23061029. VBIPsyCry128170_0842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0116.
HOGENOM	HOG000218371.
KO	K12297.
OMA	NKRGFKM.
ProtClustDB	PRK11783.
Enzyme and pathway databases
BioCyc	PCRY335284:GHE9-837-MONOMER.
Family and domain databases
HAMAP	MF_01858. 23SrRNA_methyltr_KL.
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR000241. RNA_methylase_dom. IPR017244. S-Ado-dep_RNA_MeTrfase_bac. IPR019614. SAM-dep_methyl-trfase. IPR004114. THUMP. [Graphical view]
Pfam	PF10672. Methyltrans_SAM. 1 hit. PF02926. THUMP. 1 hit. PF01170. UPF0020. 1 hit. [Graphical view]
PIRSF	PIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMART	SM00981. THUMP. 1 hit. [Graphical view]
PROSITE	PS51165. THUMP. 1 hit. PS01261. UPF0020. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMKL_PSYCK

Accession

Primary (citable) accession number: Q1QCP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program