Reviewed, UniProtKB/Swiss-Prot Q1QC40 (MAO1_PSYCK)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	Pcryo_0982

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	NAD-dependent malic enzyme HAMAP MF_01619		PRO_1000069535
Sites
	Active site	100	1	Proton donor By similarity
	Active site	171	1	Proton acceptor By similarity
	Metal binding	242	1	Divalent metal cation By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	266	1	Divalent metal cation By similarity
	Binding site	153	1	NAD By similarity
	Binding site	266	1	NAD By similarity
	Binding site	413	1	NAD By similarity
	Site	266	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QC40-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 20418FD003D94B3B	FASTA	560	62,601
	10 20 30 40 50 60 MLNQRPLYIP FAGPALLETP LLNKGSAFSS EERDSFNLTG LLPHNIETIE EQSSRAYLQL 70 80 90 100 110 120 RSFTSDMDKH IYLRNIQDTN ETLFHHLIEQ HIEEVMPLIY TPTVGQACEK FSQIYRRKRG 130 140 150 160 170 180 LFISYPERHK IDDMLQNATK QNVKVIVVTD GERILGLGDQ GIGGMGIPIG KLALYTACGG 190 200 210 220 230 240 ISPAYCLPIL LDVGTNNQQL LDDPMYMGWR NPRISGDEYN EFVDLFIQAV KRRWPEVLLQ 250 260 270 280 290 300 FEDFAQENAT PLLNKYRDQL CCFNDDIQGT AAVSVGTLIA ACLNKSQKLS QQNIAFLGAG 310 320 330 340 350 360 SAGCGIAEHI IRQMQREGLT EEQARRQVFM VDRYGLLTDS MTELQKFQTP LVQKESDIEH 370 380 390 400 410 420 WDKSQKLGLA QVVKQARITV LFGVSGQKGL FTQEVVEALC ANTEHPIVLP LSNPTSRVEA 430 440 450 460 470 480 TPQEVTNWSR GKAIVATGSP FPNTTFEGQS YEVSQCNNSY IFPGIGLGVL AARATGISDN 490 500 510 520 530 540 MLTAASQALA DISVEYEKAP GAILPPIKFI REISEKIAYA VALQAIEDKL ALPVTAENLE 550 560 RRLKANFWLP KYRDYRRTSF

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000323 Genomic DNA. Translation: ABE74763.1.
RefSeq	YP_580247.1.
3D structure databases
SMR	Q1QC40. Positions 12-560.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QC40.
Genome annotation databases
GeneID	4035727.
GenomeReviews	Gene locus Pcryo_0982 in contig CP000323_GR.
KEGG	pcr:Pcryo_0982.
NMPDR	fig|335284.3.peg.343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	IQDNNET.
PhylomeDB	Q1QC40.
Enzyme and pathway databases
BioCyc	PCRY335284:PCRYO_0982-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSYCK

Accession

Primary (citable) accession number: Q1QC40

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 16, 2006
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)